Article

[Identification of 5 constituents of the aqueous extract of Isatis indigotica by HPLC-MS2].

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 10/2005; 28(9):772-4. pp.772-4
Source: PubMed

ABSTRACT To identify five constituents in the aqueous extract of Isatis indigotica Fort.
After separation of the aqueous extract of Isatis indigotica Fort. by HPLC, the eluates of five peaks were collected separatively and analysed by MS2. UV spectra and MS2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine.
Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS2 spectrum as the corresponding standards.
Five constituents of the aqueous extract of Isatis indigotica Fort. are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine.

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Keywords

analysed
 
aqueous
 
cytidine
 
fragment pattern
 
guanosine
 
guanosine xanthine
 
HPLC
 
HPLC elute
 
hypoxanthine
 
Isatis indigotica Fort
 
MS2 spectrum
 
peaks
 
reference standards
 
retention time
 
uridine
 
UV spectra