Article

Catellicoccus marimammalium gen. nov., sp. nov., a novel Gram-positive, catalase-negative, coccus-shaped bacterium from porpoise and grey seal.

Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA.
International journal of systematic and evolutionary microbiology (impact factor: 2.27). 03/2006; 56(Pt 2):429-32. DOI:10.1099/ijs.0.63874-0 pp.429-32
Source: PubMed

ABSTRACT Two strains of an unknown Gram-positive, catalase-negative, facultatively anaerobic, coccus-shaped bacterium, originating from a porpoise and a grey seal, were characterized using phenotypic, biochemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on L-lysine (type L-lys-gly-D-Asp) and a DNA G+C content of 38 mol%. Comparative 16S rRNA gene sequencing showed that this unidentified coccus-shaped organism formed a hitherto unknown subline closely related to, albeit distinct from, a number of other catalase-negative genera which included Enterococcus, Melissococcus, Tetragenococcus and Vagococcus. Other known Gram-positive, catalase-negative taxa were more distantly related. Tree-branching considerations and sequence divergence values of >6% with recognized taxa were indicative of this novel bacterium representing a separate genus. Based on phenotypic and phylogenetic evidence, it is proposed that this unknown bacterium, recovered from a porpoise and a grey seal, be classified as a novel genus and species, Catellicoccus marimammalium gen. nov., sp. nov. The type strain is M35/04/3T (=CCUG 49459T=CIP 108575T).

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Keywords

catalase-negative genera
 
catalase-negative taxa
 
Catellicoccus marimammalium gen
 
cell-wall murein
 
Chemical studies
 
Comparative 16S rRNA gene sequencing
 
DNA G+C content
 
grey seal
 
known Gram-positive
 
molecular phylogenetic methods
 
novel bacterium
 
novel genus
 
phylogenetic evidence
 
separate genus
 
sequence divergence values
 
strains
 
type L-lys-gly-D-Asp
 
type strain
 
unknown Gram-positive
 
unknown subline