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High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study.

M Aires de Sousa, K Boye, H de Lencastre, A Deplano, M C Enright, J Etienne, A Friedrich, D Harmsen, A Holmes, X.W. Huijsdens, A M Kearns, A Mellmann, H Meugnier, J K Rasheed, E. Spalburg, B Strommenger, M J Struelens, F C Tenover, J Thomas, U Vogel, H Westh, J Xu, W Witte

Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany.

Journal Article: Journal of Clinical Microbiology (impact factor: 4.16). 03/2006; 44(2):619-21. DOI: 10.1128/JCM.44.2.619-621.2006

Abstract

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

Source: PubMed

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Keywords

10 laboratories
 
30 blind-coded S. aureus DNA samples
 
common typing nomenclature
 
Current DNA amplification-based typing methods
 
DNA sequence-based typing
 
extensive harmonization
 
international study
 
sequence analysis ensured