Evaluation of the CombiChip Mycobacteria (TM) Drug-Resistance detection DNA chip for identifying mutations associated with resistance to isoniazid and rifampin in Mycobacterium tuberculosis
Pusan National University, Tsau-liang-hai, Busan, South KoreaDiagnostic Microbiology and Infectious Disease (Impact Factor: 2.46). 04/2006; 54(3):203-10. DOI: 10.1016/j.diagmicrobio.2005.09.014
The CombiChip Mycobacteriatrade mark Drug-Resistance Detection DNA chip, recently developed by GeneIn (Pusan, South Korea), is an oligonucleotide microchip coupled with polymerase chain reaction for the detection of mutations associated with resistance to isoniazid (INH) and rifampin (RIF). This oligonucleotide chip was compared with DNA sequencing and phenotypic drug susceptibility testing with 69 INH- and/or RIF-resistant and 27 all tested drug-susceptible Mycobacterium tuberculosis isolates. Two selected codons (the katG codon 315 and inhA15) allowed identification of 84.1% of INH-resistant isolates and 100% of RIF resistance were detected by screening for 7 codons: rpoB511, rpoB513, rpoB516, rpoB522, rpoB526, rpoB531, and rpoB533. The overall specificity of this oligonucleotide chip for detecting INH and RIF resistance were 100 and 95.3%, respectively. This level of sensitivity and specificity is concordant with that from the determination of M. tuberculosis drug resistance by DNA sequencing. This oligonucleotide chip is a rapid and reliable genotypic method capable of detecting multiple mutations associated with INH and RIF resistance simultaneously in a single microchip slide.
Article: Genotypic Drug Resistance Assays[Show abstract] [Hide abstract]
ABSTRACT: The purpose of this review is to describe the mechanisms of antimicrobial resistance and to present some genotypic drug resistance assays used to detect antimicrobial resistance. Genotypic drug resistance assays that are increasingly used in the clinical microbiology laboratory and their applications in the clinical settings will be further discussed.
Conference Paper: Microwave propagation over a mountain-diffraction path[Show abstract] [Hide abstract]
ABSTRACT: Stanford University has been conducting a theoretical-experimental study of obstacle diffraction at microwave frequencies. The experimental program utilizes Mt. Diablo, 36 miles northeast of Stanford, with a height of 3850 feet, as the diffracting obstacle. The path profile is shown in Figure 1. The core of the program is frequency-sweep transmission which permits time-shared study of received power as a function of both frequency and time. The transmitter, a 300 watt carcinotron, is frequency-modulated by a 25 cps sawtooth waveform over a 60 Mc range centered at 3130 Mc. The receiver is swept in a similar fashion once per second. The resulting video display consists of 25 "pulses" per second corresponding to each frequency interception, with a complete scan of the frequency range each second. This pattern is recorded on magnetic tape for later playback and for analysis. Line-of-sight measurements, for calibration purposes, show the system response to be flat to within pm 1 db.Antennas and Propagation Society International Symposium, 1963; 08/1963
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ABSTRACT: The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validated by using 78 reference strains belonging to 55 Mycobacterium species, 219 clinical isolates which had been identified as mycobacteria by high-performance liquid chromatography or gas chromatography, three skin biopsy specimens from patients with suspected leprosy which had been shown to contain acid-fast bacilli, and isolates of 14 nonmycobacterial species. All mycobacteria were amplified in the PCR and hybridized with a genus-specific probe (probe MYC). The 34 species-specific probes designed in this study hybridized only with the corresponding Mycobacterium species. The mycobacterial PCR-RLB assay is an efficient tool for the identification of clinical isolates of mycobacteria; it can reliably identify mixed mycobacterial cultures and M. leprae in skin biopsy specimens.Journal of Clinical Microbiology 11/2006; 44(10):3544-50. DOI:10.1128/JCM.00633-06 · 3.99 Impact Factor
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