Article

HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies.

School of Pharmacy, Second Military Medical University, Shanghai Key Laboratory for Pharmaceutical Metabolites Research, No. 325, Guohe Road, Shanghai 200433, PR China.
Journal of Pharmaceutical and Biomedical Analysis (impact factor: 2.97). 04/2006; 41(1):240-5. DOI:10.1016/j.jpba.2005.08.027 pp.240-5
Source: PubMed

ABSTRACT A reversed-phase high-performance liquid chromatographic (RPHPLC) method has been developed for the determination of polydatin (PD) in rat plasma, bile, urine, feces and tissue homogenates using 2,3,5,4'-tetrahydroxychrysophenine-beta-d-glucoside as an internal standard. The sample pretreatment included deproteinization for plasma samples and a liquid-liquid extraction for bile, urine, feces and tissue homogenates. Separation was obtained on a C18 reversed-phase column with the mobile phase consisting of methanol and water (35:65 v/v). The flow rate was 1 ml/min and the effluent was monitored at 310 nm. The method showed good linearity over the concentration ranges employed for various matrices (r > 0.998). The quantification limits of PD in rat plasma, bile, urine, feces and tissue homogenates were 0.0251, 0.126, 0.025 microg/ml, 0.189 and 0.0378 microg/g, respectively. The accuracy and precision of the method were less than 12.0% for the various matrices. No interferences from endogenous substances were found. The method was successfully applied to study the pharmacokinetics of PD in rats after intravenous administration.

0 0
 · 
0 Bookmarks
 · 
7 Views
  • Source
    Article: Polydatin up-regulates Clara cell secretory protein to suppress phospholipase A2 of lung induced by LPS in vivo and in vitro.
    Shu Shiyu, Ling Zhiyu, Ye Mao, Bo Lin, Wang Lijia, Zhang Tianbao, Chen Jie, Li Tingyu
    [show abstract] [hide abstract]
    ABSTRACT: Lung injury induced by lipopolysaccharide (LPS) remains one of the leading causes of morbidity and mortality in children. The damage to membrane phospholipids leads to the collapse of the bronchial alveolar epithelial barrier during acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Phospholipase A2 (PLA2), a key enzyme in the hydrolysis of membrane phospholipids, plays an important traumatic role in pulmonary inflammation, and Clara cell secretory protein (CCSP) is an endogenous inhibitor of PLA2. Our previous study showed that polydatin (PD), a monocrystalline extracted from a traditional Chinese medicinal herb (Polygonum cuspidatum Sieb, et Zucc), reduced PLA2 activity and sPLA2-IIA mRNA expression and mitigated LPS-induced lung injury. However, the potential mechanism for these effects has not been well defined. We have continued to investigate the effect of PD on LPS-induced expression of CCSP mRNA and protein in vivo and in vitro. Our results suggested that the CCSP mRNA level was consistent with its protein expression. CCSP expression was decreased in lung after LPS challenge. In contrast, PD markedly increased CCSP expression in a concentration-dependent manner. In particular, CCSP expression in PD-pretreated rat lung was higher than in rats receiving only PD treatment. These results indicated that up-regulation of CCSP expression causing inhibition of PLA2 activation may be one of the crucial protective mechanisms of PD in LPS-induced lung injury.
    BMC Cell Biology 07/2011; 12:31. · 2.59 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

2,3,5,4'-tetrahydroxychrysophenine-beta-d-glucoside
 
C18 reversed-phase column
 
concentration ranges
 
interferences
 
internal standard
 
intravenous administration
 
liquid-liquid extraction
 
mobile phase
 
PD
 
pharmacokinetics
 
plasma samples
 
quantification limits
 
rats
 
reversed-phase high-performance liquid chromatographic
 
sample pretreatment
 
tissue homogenates
 
various matrices