Effects of Xuebijing injection on multiple organ dysfunction and mortality in rats with delayed resuscitation for scald injury

Burns Institute, First Affiliated Hospital of General Hospital of PLA, Beijing 100037, China.
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 01/2006; 18(1):16-8.
Source: PubMed


To investigate the effects of Xuebijing injection on multiple organ dysfunction and mortality in rats with delayed resuscitation after scald injury.
Wistar rats were subjected to a 30% total body surface area (TBSA) full-thickness scald injury in the experimental groups. One hundred and thirty male Wistar rats were randomly divided into sham scald group (n=10), thermal injury group (n=60, 40 ml/kg normal saline was infused peritoneally 6 hours postburn), and Xuebijing treatment group (n=60, 4 ml/kg Xuebijing injection was injected twice a day in addition to delayed resuscitation). Animals of the thermal injury group and Xuebijing treatment group were re-divided into three subgroups according to the different time points of treatment: 2 hours before scald (n=20), 2 hours after scald (n=20), and 12 hours after scald (n=20). Multiple organ function parameters and 7-day mortality were determined in all the groups.
The mortality of animals with Xuebijing treatment at 12 hours postburn was significantly lower than that of scald controls (75.0% vs. 40.0%, P<0.05). In comparison with scald group, the animals in Xuebijing treatment group were found to have significantly decreased values of organ functional parameters at 12 hours postburn, including serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr) and creatine kinase (CK, P<0.05 or P<0.01).
These findings indicate that Xuebijing injection could obviously lower the mortality of rats with delayed resuscitation after burn injury, and it markedly protects against multiple organ dysfunction secondary to severe burns.

0 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: Interleukin (IL)-23 has been identified as a member of the IL-12 cytokine family. It plays an important role in inflammation. To demonstrate the changes of IL-23 in acute lung injury (ALI) and investigate the protective effect of Xuebijing in ALI and the underlying molecular mechanism, ALI was induced by intravenous injection of lipopolysaccharide (LPS, 750 microg/kg). Japanese white rabbits challenged with or without LPS were treated with Xuebijing at the same time or saline. Before and after administration of LPS, arterial blood gas and lung weight gain were examined. Pathological changes of lung tissue were measured by light microscopy. IL-23 in serum was detected by enzyme-linked immunosorbent assay (ELISA). All animals demonstrated drops in arterial oxygen tension (Pao(2)) and oxygenation index (Pao(2)/Fio(2)) after LPS application, which were significantly reversed by Xuebijing treatment. Administration of Xuebijing reduced lung water gain. Histopathological study also indicated that Xuebijing treatment markedly attenuated lung histopathological changes, alveolar hemorrhage and inflammatory cells infiltration. Furthermore, IL-23 was higher than control group after LPS treatment, which could be blunted by Xuebijing. These findings confirmed significant protection by Xuebijing against LPS-induced lung vascular leak and inflammation and implicated inhibition of IL-23 expression a potential role for Xuebijing in the management of ALI.
    Experimental Lung Research 05/2010; 36(4):211-8. DOI:10.3109/01902140903312123 · 1.41 Impact Factor