Sequencing and staphylococci identification.

Alexander Mellmann, Karsten Becker, Christof von Eiff, Ursula Keckevoet, Peter Schumann, Dag Harmsen

University Hospital Münster, Münster, Germany.

Journal Article: Emerging infectious diseases (impact factor: 6.79). 03/2006; 12(2):333-6.

Abstract

The emerging clinical importance of staphylococcal infections prompted us to establish a reference database for partial RNA polymerase B (rpoB; nucleotides 1444-1928) gene sequences from type strains of all staphylococcal species and subspecies. This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates.

Source: PubMed

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Keywords

emerging clinical importance
 
partial RNA polymerase B
 
reference database
 
rpoB
 
subspecies
 
subspecies level