Acidic polysaccharide from Phellinus linteus inhibits melanoma cell metastasis by blocking cell adhesion and invasion.
ABSTRACT The acidic polysaccharide (PL) from Phellinus linteus is an immunostimulator that has therapeutic activity against cancers. Here, we show that PL markedly inhibits melanoma cell metastasis in mice, and report that PL directly inhibits cancer cell adhesion to and invasion through the extracellular matrix, but that it has no direct effect on cancer cell growth. In addition, we found that PL increased macrophage NO production. These results suggest that PL has two antimetastatic functions, i.e., it acts as an immunopotentiator and as a direct inhibitor of cancer cell adhesion.
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ABSTRACT: Phellinus linteus (Sang Hwang mushroom; P. linteusis) has been widely used for a traditional medicine in Japan, Korea and China. In traditional oriental applications, P. linteus and their extracts have been exclusively used to treat various diseases like insomnia, neurasthenia, gastric ulcers, arthritis, nephritis, stress, asthma, bronchitis, neuromuscular disorders, hypertension and also as an immune system stimulator in cancer therapy. In a number of articles, it is indicated how tremendous their therapeutic values are. According to these studies, P. linteus and their extracts show immunomodulatory activities like enhancing immune system function including anti-tumor activity and inhibiting the growth of existing tumors. P. linteus and their extracts have been being studied for use as immunotherapy agents and biological response modifiers (BRMs) for the possible treatment of cancer without side effects. Recently, the cultured mycelium product of P. linteus has been developed and approved as a medicine in Korea. This review shows not only the current status of methods of developmental technology like artificial culture and various biological applications of P. linteus but also recent market trends of functional foods made from P. linteus.KSBB Journal. 01/2008; 23(2).
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ABSTRACT: There have been relatively few direct intervention trials of mushroom consumption in humans, although those that have been completed to date indicate that mushrooms and their extracts are generally well-tolerated with few, if any, side-effects. Immunomodulating and anti-tumor effects of mushrooms and their extracts appear to hold potential health benefits. These benefits are primarily due to their polysaccharide content, either in the form of beta-glucans or polysaccharide-protein complexes, which appear to exert their anti-tumorigenic effects by enhancement of cellular immunity via effects on the balance of T helper cell populations and induction of certain interleukins and interferon (IFN)-γ. This review summarizes the current knowledge on edible mushrooms and their components on health outcomes, with a focus on the evaluation of the evidence from human trials. Where information is available from such trials, the active compounds are identified and their proposed mechanisms are discussed.Journal of Functional Foods 10/2012; 4(4):687-709. · 4.48 Impact Factor
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ABSTRACT: The aim of this work was to optimize the submerged culture medium for exopolysaccharides (EPS) production from Inocutis tamaricis (Pat.) Fiasson & Niemelä. The molecular characterization and the evaluation of antioxidant activity and antitumor activity in vitro of EPS were carried out as well. The one-factor-at-a-time method was adopted to investigate the optimal initial pH and temperature as 8.0 and 25 °C, respectively. The optimal culture condition was obtained as follows: sucrose 64.09 g/L and tryptone 5.24 g/L by central composite design (CCD). Under optimal culture condition, the maximum EPS concentration in shake flask was 5.89 g/L. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 8.948 × 104 and 6.421 × 104 g/mol, respectively. The SEC/MALLS analysis revealed that the molecular shapes of the Fr-I and Fr-II were the rigid sphere suspected to be the aggregates of complex polysaccharides. Furthermore, Fr-II was exhibited higher antioxidant and antitumour activities than Fr-I. Finally, FT-IR spectroscopy was used for obtaining vibrational spectra of the Fr-II EPS and the obvious absorption peaks at 916.5 and 884.0 cm−1 revealed the co-existence of α and β configuration. The carbohydrate composition analysis revealed Fr-II EPS was mainly composed of mannose, glucose and talose.Journal of the Taiwan Institute of Chemical Engineers 05/2014; 45(3):725–733. · 2.64 Impact Factor