Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group.

Department of Chemistry, Graduate School of Science, Tohoku University, Japan.
Analytical Sciences (Impact Factor: 1.57). 03/2006; 22(2):201-3. DOI: 10.2116/analsci.22.201
Source: PubMed

ABSTRACT The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M(-1) of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.