Article

Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling machines

Boston University, Boston, Massachusetts, United States
Journal of Proteome Research (Impact Factor: 5). 04/2006; 5(3):502-11. DOI: 10.1021/pr050430u
Source: PubMed

ABSTRACT We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.

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