Aislabie J, Saul DJ, Foght JM.. Bioremediation of hydrocarbon-contaminated polar soils. Extremophiles 10: 171-179

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Extremophiles (Impact Factor: 2.31). 07/2006; 10(3):171-9. DOI: 10.1007/s00792-005-0498-4
Source: PubMed


Bioremediation is increasingly viewed as an appropriate remediation technology for hydrocarbon-contaminated polar soils. As for all soils, the successful application of bioremediation depends on appropriate biodegradative microbes and environmental conditions in situ. Laboratory studies have confirmed that hydrocarbon-degrading bacteria typically assigned to the genera Rhodococcus, Sphingomonas or Pseudomonas are present in contaminated polar soils. However, as indicated by the persistence of spilled hydrocarbons, environmental conditions in situ are suboptimal for biodegradation in polar soils. Therefore, it is likely that ex situ bioremediation will be the method of choice for ameliorating and controlling the factors limiting microbial activity, i.e. low and fluctuating soil temperatures, low levels of nutrients, and possible alkalinity and low moisture. Care must be taken when adding nutrients to the coarse-textured, low-moisture soils prevalent in continental Antarctica and the high Arctic because excess levels can inhibit hydrocarbon biodegradation by decreasing soil water potentials. Bioremediation experiments conducted on site in the Arctic indicate that land farming and biopiles may be useful approaches for bioremediation of polar soils.

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    • "The effectiveness of bioremediation strategies has been reported for soils in cold regions (Aislabie et al, 2006; Sanscartier et al, 2009). In previous studies, we have shown the effectiveness of bioremediation techniques to reduce hydrocarbon contamination in Antarctic soils (Mac Cormack and Fraile, 1997; Ruberto et al., 2003, 2006, 2009; Vázquez et al., 2009). "
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    ABSTRACT: Bioremediation is a biotechnological approach to clean up contaminated soils. Among bioremediation strategies, biostimulation is a simple method which involves the modification of the soil physicochemical conditions in order to enhance the biological degradation of contaminants. One of the most common ways to do this is by the addition of macronutrients, mainly Nitrogen (N) and Phosphorus (P). Optimization of the amounts of N and P for a soil biostimulation strategy represents a key step prior to its application to a full-scale process. In this work, the response-surface methodology (RSM) was applied to optimize a biostimulation process for a hydrocarbon-contaminated Antarctic soil, considering a Carbon:Nitrogen:Phosporus (C:N:P) ratio of 100:10:1 as a reference. A faced-centered central composite design was used to determine the levels of the variables that lead to the optimum response values. Flasks containing contaminated soil and receiving different N and P amounts were incubated at 15 °C for 80 days. Biological activity and hydrocarbon concentration were evaluated. Results predicted that for the soil used in this experiment, the addition of 0.183 g N/kg and 0.0179 g P/kg leads to the highest hydrocarbon removal efficiency. The resulting C:N:P ratio (100:17.6:1.73) was different from that taken as reference (100:10:1), highlighting the usefulness of such an optimization. The hydrocarbon concentration decreased from 1042 (±73) mg kg−1 to 470 (±37) mg kg−1 in the most efficient combination tested.
    Cold Regions Science and Technology 07/2015; 119:61-67. DOI:10.1016/j.coldregions.2015.07.005 · 1.37 Impact Factor
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    • "Wherever petroleum is found in freezing and frozen soils, they can be degraded by hydrocarbon-degrading microorganisms [9]–[11]. Cold-adapted intrinsic bacteria can be still active in cold regions and have potential to in-situ break down petroleum pollutants, even though they are influenced by environmental limitation [11]. Most research has considered hydrocarbon degradation in the active layer, while a substantial number of hydrocarbon degraders have also been detected in permafrost soils [12]. "
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    ABSTRACT: The buried China-Russia Crude Oil Pipeline (CRCOP) across the permafrost-associated cold ecosystem in northeastern China carries a risk of contamination to the deep active layers and upper permafrost in case of accidental rupture of the embedded pipeline or migration of oil spills. As many soil microbes are capable of degrading petroleum, knowledge about the intrinsic degraders and the microbial dynamics in the deep subsurface could extend our understanding of the application of in-situ bioremediation. In this study, an experiment was conducted to investigate the bacterial communities in response to simulated contamination to deep soil samples by using 454 pyrosequencing amplicons. The result showed that bacterial diversity was reduced after 8-weeks contamination. A shift in bacterial community composition was apparent in crude oil-amended soils with Proteobacteria (esp. α-subdivision) being the dominant phylum, together with Actinobacteria and Firmicutes. The contamination led to enrichment of indigenous bacterial taxa like Novosphingobium, Sphingobium, Caulobacter, Phenylobacterium, Alicylobacillus and Arthrobacter, which are generally capable of degrading polycyclic aromatic hydrocarbons (PAHs). The community shift highlighted the resilience of PAH degraders and their potential for in-situ degradation of crude oil under favorable conditions in the deep soils.
    PLoS ONE 05/2014; 9(5):e96552. DOI:10.1371/journal.pone.0096552 · 3.23 Impact Factor
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    • "Nevertheless, excess of nutrients could also reduce microbial activity and loss of hydrocarbons (e.g., Braddock et al. 1997). Despite bioremediation of PHC by indigenous coldadapted microorganisms being reported at low temperatures (Aislabie et al. 2006; Rike et al. 2003), they also persist in contaminated soils (Aislabie et al. 2004). Temperature from 1 to above 10 °C (e.g., Chang et al. 2010; Chang et al. 2011; Delille et al. 2007; Ferguson et al. 2008) increased degradation and/or its ratio. "
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    ABSTRACT: A 168-day period field study, carried out in Sisimiut, Greenland, assessed the potential to enhance soil remediation with the surplus heating from an incineration facility. This approach searches a feasible ex situ remediation process that could be extended throughout the year with low costs. Individual and synergistic effects of biostimulation were also tested, in parallel. An interim evaluation at the end of the first 42 days showed that biostimulation and active heating, as separate treatments, enhanced petroleum hydrocarbon (PHC) removal compared to natural attenuation. The coupling of both technologies was even more effective, corroborating the benefits of both techniques in a remediation strategy. However, between day 42 and day 168, there was an opposite remediation trend with all treatments suggesting a stabilization except for natural attenuation, where PHC values continued to decrease. This enforces the "self-purification" capacity of the system, even at low temperatures. Coupling biostimulation with active heating was the best approach for PHC removal, namely for a short period of time (42 days). The proposed remediation scheme can be considered a reliable option for faster PHC removal with low maintenance and using "waste heating" from an incineration facility.
    Environmental Science and Pollution Research 02/2014; 21(9). DOI:10.1007/s11356-013-2466-3 · 2.83 Impact Factor
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