Article

Genomic features of attenuated Junín virus vaccine strain candidate.

LIGBCM, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Rogue Saenz Peña 180, B1876BXD, Bernal, Buenos Aires, Argentina.
Virus Genes (Impact Factor: 1.84). 03/2006; 32(1):37-41. DOI: 10.1007/s11262-005-5843-2
Source: PubMed

ABSTRACT Junin virus strain Candid #1 was developed as a live attenuated vaccine for Argentine haemorrhagic fever. In this paper, we report the nucleotide sequences of L RNA of Candid #1 and examine the relationship to its more virulent ancestors Junin virus XJ#44 and XJ 13 (prototype) and other closely and distantly related arenaviruses. Comparisons of the nucleotide and amino acid sequences of L and Z genes of Candid #1 and its progenitor strains revealed twelve point mutations in the L polypeptide that are unique to the vaccine strain. These changes could be provisionally associated with the attenuated phenotype. In contrast, Z ORF was completely conserved among all strains.

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    • "In this work, we show the strategies employed for the expression, purification, and specific antibody generation against Z protein from Candid#1 strain of Junín virus [21]. Here we report the optimized expression from a synthetic gene of Z protein tagged with different peptides, using three expression systems (two bacterial and a baculoviral one), in order to obtain recombinant Z protein suitable for functional characterization studies. "
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