Cultures of rat olfactory ensheathing cells are contaminated with Schwann cells.
ABSTRACT Implantation of cultured olfactory ensheathing cells into the damaged spinal cord of adult rats has been reported to remyelinate central axons. This observation is curious because olfactory ensheathing cells do not myelinate axons in their native environment. We have recently determined that calponin is the first definitive phenotypic marker for olfactory ensheathing cells. Primary cultures of adult rat olfactory mucosa and olfactory bulb were immunostained for p75 neurotrophin receptor and calponin. Our results reveal that two populations of p75 neurotrophin receptor-positive cells exist in primary cultures of the olfactory mucosa and bulb: calponin-positive olfactory ensheathing cells and calponin-negative Schwann cells. As olfactory tissues likely yield a mixed glial population, the idea that olfactory ensheathing cells are capable of de novo myelin synthesis after intraspinal implantation should be re-evaluated.
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ABSTRACT: We have studied the charge loss in WSi2 nanocrystals nonvolatile memory device with silicon oxide-nitride-oxide (SiO2: 2 nm/Si3N4:2 nm/SiO2: 3 nm) tunnel layer. The WSi2 nanocrystals of 2.5 nm diameters and 3.6 × 1012 cm−2 density were formed between tunnel and control oxide layers. When the programming/erasing voltages were applied at 10 V/−10 V during 500 ms, the memory window was measured about 2.7 V and maintained at about 1.1 V after 104 s at 25 °C. In this device, the activation energies for the charge loss rates from 10% to 50% in compare to an initial charge were about 0.14 eV. This charge loss could be caused by a cycling-induced oxide damage or tunnel oxide break down. Therefore, it has a feasibility of application to highly-integrate nonvolatile memory after optimize the charge loss effect by thermal stress and improve the tunnel layer stability.Current Applied Physics 03/2011; 11(2):e6–e9. DOI:10.1016/j.cap.2010.12.036 · 2.03 Impact Factor
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ABSTRACT: The aim of the present study was to investigate the effects of human umbilical cord blood-derived mesenchymal stem cell (HUCB-MSC) transplantation on the functional restoration of spinal cord injury (SCI). A total of 46 adult Wistar rats were randomly divided into three groups: Injury (n=15), control (n=15) and transplantation (n=16). A SCI model was established using the modified Allen's method (vulnerating energy, 25 g/cm). The rats in the control and transplantation groups were injected at the site of the injury with physiological saline and HUCB-MSC suspension, respectively. At week one, two and four following treatment, the behavior of the rats was evaluated using the Basso, Beattie, Bresnahan locomotor rating scale. In addition, immunohistochemistry (IHC) was performed on samples from the rats that had been sacrificed four weeks subsequent to the treatment. Recovery of the spinal cord nerve function was identified to be significantly different at week two and four following treatment (P<0.05), and IHC identified that at week four following treatment novel nerve cells were being produced. Thus, transplantation of HUCB-MSCs promoted the recovery of the damaged function of spinal cord nerves in rats with SCI.Experimental and therapeutic medicine 05/2014; 7(5):1233-1236. DOI:10.3892/etm.2014.1608 · 0.94 Impact Factor