Article

Removal of contaminating TEM-la beta-lactamase gene from commercial Taq DNA polymerase.

Department of Biological Sciences, Myongji University, San 38-2 Namdong, Yongin, Gyeonggido 449-728, Republic of Korea.
The Journal of Microbiology (Impact Factor: 1.28). 03/2006; 44(1):126-8.
Source: PubMed

ABSTRACT This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

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May 15, 2014