Article

[Analysis of molecular profiles among Trypanozoon species and subspecies by MGE-PCR method].

Center for Parasitic Organisms, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou, China.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 11/2005; 23(5):277-82. pp.277-82
Source: PubMed

ABSTRACT To analyze the relationship between genetic variability and evolution among Trypanosoma brucei (including T. b. brucei, T. b. rhodesiense and T. b. gambiense), T. evansi and T. equiperdum isolates.
Genomic DNAs of 26 trypanosome isolates were amplified by a mobile genetic elements (MGE) -PCR technique and cluster analysis was performed based on the molecular profiles with Neighbor-Joining method.
The genetic variability among trypanosome isolates examined was obvious with an average genetic distance of 41.2% (ranged from 0 to 100%). Similarity coefficient among T. brucei isolates was 41.15% which was lower than that between T. evansi and T. equiperdum isolates. The closest relationship was found between T. evansi and T. brucei isolates with a similarity coefficient of 62.94%. The genetic variability between T. b. rhodesiense and T. b. brucei isolates was higher than that among T. b. gambiense isolates.
Species and subspecies in Trypanozoon displayed a higher genetic variability; T. equiperdum isolates collected from China and from South America, and T. evansi isolates from China and from South America, should have a similar origin.

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Keywords

26 trypanosome
 
average genetic distance
 
cluster analysis
 
genetic variability
 
Genomic DNAs
 
higher genetic variability
 
mobile genetic elements
 
molecular profiles
 
similar origin
 
similarity coefficient
 
South America
 
subspecies
 
T. b. brucei
 
T. b. gambiense
 
T. b. rhodesiense
 
T. brucei
 
T. equiperdum
 
T. evansi
 
Trypanosoma brucei
 
trypanosome