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[Diversity of halophilic archaea in hypersaline lakes of Inner Mongolia, China].

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
ACTA MICROBIOLOGICA SINICA 03/2006; 46(1):1-6.
Source: PubMed

ABSTRACT The aims of this work were to explore the diversity of halophilic archaea in hypersaline lakes of Inner Mongolia, China and to collect novel halophilic archaea. One hundred and sixty-five halophilic archaea were isolated from the three different types of hypersaline lakes (Erliannor, shangmatala and Xilin soda lake) in Inner Mongolia. By analysis of the restriction patterns of amplified 16S rDNA (ARDRA) with the enzyme Afa I and Hae II, respectively, the isolates were clustered into 14 genotypes, and the representatives of each genotype were randomly chosen for the determination of 16S rDNA sequence. The phylogenetic analysis revealed that all of the isolates were clustered into 10 groups: Halorubrum, Natronococcus, Natronorubrum, Haloterrigena, Halorhabdus, Halobiforma, Haloarcula, Haloferax and other two unknown groups. Dominant isolates were related to Halorubrum spp. in all three lakes. Some of the isolates studied showed less affiliation with known taxa ( <98% sequence similarity) and may represent novel taxa. Two isolates HXH33 and HSH33 showed very less affiliation with known genus ( < 93% sequence similarity) and may represent two new genera. These results suggest that diverse archaea exist in and the unknown archaea thrive in the hypersaline lakes of Inner Mongolia.

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    • "Recently, Natronorubrum aibiense was added to the genus (Cui et al., 2006a). Members of the genus Natronorubrum have been frequently isolated from several salt lakes in China (Fan et al., 2003; Pan et al., 2006). The Aiding salt lake (42u 329 100–42u 499 130 N 89u 109 320–89u 549 320 E), the lowest point in China (155 m below sea-level) and after the Dead Sea the second lowest inland depression in the world, has been a target for the study of halophilic archaeal diversity under extremely high salt conditions for many years (Tohty & Xu, 2001; Cui et al., 2006b). "
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    International Journal of Systematic and Evolutionary Microbiology 04/2007; 57(Pt 4):738-40. DOI:10.1099/ijs.0.64651-0 · 2.80 Impact Factor
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    • "Due to the biotechnological importance of new molecules from halophiles, the intensive work with isolation of new strains from hyper-saline ecosystems continues. One hundred and sixtyfive halophilic archaea were isolated from three different types of hypersaline lakes (Erliannor, Shangmatala, and Xilin soda lake) in Inner Mongolia (Pan et al. 2006). "
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    Critical Reviews in Microbiology 02/2007; 33(3):183-209. DOI:10.1080/10408410701451948 · 6.09 Impact Factor
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    • "Due to the biotechnological importance of new molecules from halophiles, the intensive work with isolation of new strains from hyper-saline ecosystems continues. One hundred and sixtyfive halophilic archaea were isolated from three different types of hypersaline lakes (Erliannor, Shangmatala, and Xilin soda lake) in Inner Mongolia (Pan et al. 2006). "
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