Multiplexed Protein Measurement: Technologies and Applications of Protein and Antibody Arrays

National Center for Genome Resources, Santa Fe, New Mexico 87505, USA.
Nature Reviews Drug Discovery (Impact Factor: 41.91). 05/2006; 5(4):310-20. DOI: 10.1038/nrd2006
Source: PubMed


The ability to measure the abundance of many proteins precisely and simultaneously in experimental samples is an important, recent advance for static and dynamic, as well as descriptive and predictive, biological research. The value of multiplexed protein measurement is being established in applications such as comprehensive proteomic surveys, studies of protein networks and pathways, validation of genomic discoveries and clinical biomarker development. As standards do not yet exist that bridge all of these applications, the current recommended best practice for validation of results is to approach study design in an iterative process and to integrate data from several measurement technologies. This review describes current and emerging multiplexed protein measurement technologies and their applications, and discusses the remaining challenges in this field.

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Available from: Stephen F Kingsmore, Jan 04, 2014
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    • "Multiplex immunoassay (MIA) use allows the measurement of dozens of cytokines, chemokines, and adhesion molecule protein concentration in brain extracts from both patients and experimental animals. This technological breakthrough allows the visualization of complex networks of interacting cytokines [23] [24] [25]. "
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    Epilepsy & Behavior 09/2014; 38. DOI:10.1016/j.yebeh.2014.01.017 · 2.26 Impact Factor
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