High-throughput RNA interference in functional genomics

Department Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Fabeckstr. 60-62, 14195 Berlin, Germany.
Handbook of experimental pharmacology 02/2006; 173(173):97-104. DOI: 10.1007/3-540-27262-3_5
Source: PubMed


RNA interference (RNAi) refers to post-transcriptional silencing of gene expression as a result of the introduction of double-stranded RNA into cells. The application of RNAi in experimental systems has significantly accelerated elucidation of gene functions. In order to facilitate large-scale functional genomics studies using RNAi, several high-throughput approaches have been developed based on microarray or microwell assays. The recent establishment of large libraries of RNAi reagents combined with a variety of detection assays has further improved the performance of functional genome-wide screens in mammalian cells.

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