Yellow fever vaccine-associated viscerotropic disease and death in Spain.
ABSTRACT Yellow fever vaccine-associated viscerotropic disease (YEL-AVD) is a recently described severe adverse event after yellow fever vaccination, and some cases have been reported in different countries [Anonymous. Effects of yellow fever and vaccination. Lancet 2001;358(9296):1907-9]. Herein we describe a YEL-AVD case in a young woman, who died after vaccination with 17D-204 strain. Clinical, serological and immunochemical analysis as well as virus detection, quantification, sequence analysis and cytokine release, were performed. Further investigations on yellow fever vaccine adverse events, and carefully analysis of the immune response elicited are important tasks for the future.
- SourceAvailable from: Stefan S Biel[show abstract] [hide abstract]
ABSTRACT: Yellow fever virus quantitation is performed routinely by cultivation of virus containing samples using susceptible cells. Counting of the resulting plaques provides a marker for the number of infectious particles present in the sample. This assay usually takes up to 5 days before results are obtained and must be carried out under L2 or L3 laboratory conditions, depending on the yellow fever virus strain used. For clinical diagnosis of yellow fever virus infections the cell culture-based approach takes too long and is of limited practical relevance. Recently, due to its considerable sensitivity, PCR has become a promising method for virus detection. However, whilst PCR can detect virus-specific nucleic acids, it does not allow conclusions to be drawn regarding the infectious potential of the virus detected. Nonetheless, for diagnostic purposes, a rapid, specific and sensitive virus PCR is preferable. Therefore, two independent yellow fever virus-specific real-time PCR assays were established and compared the viral RNA loads to the results of a traditional plaque assay. The estimated ratio of yellow fever virus genomes to infectious particles was between 1000:1 and 5000:1; both approaches displayed a comparable precision of <45%. A significant correlation between genome number as determined by real-time PCR and the corresponding number of plaques in paired samples was found with a Pearson coefficient of correlation of r=0.88 (P<0.0001).Journal of Virological Methods 06/2003; 110(2):185-91. · 1.90 Impact Factor
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ABSTRACT: Flaviviruses are a widespread and numerous group of arboviruses that can cause serious illness in humans. The continuous and slow spread of certain flaviviruses, such as Dengue viruses, and the recent entry and spread of West Nile virus to the American continent, point to the need to control these infections. This control requires the use of suitable techniques for diagnostic and surveillance programmes. A generic RT-nested-PCR that is, theoretically, able to detect each member of the group has been designed. The identification of the detected virus is carried out by sequencing. The introduction of an internal control would reduce the number of false negative results and could be used to quantify the viral load in clinical samples where the method works well.Journal of Virological Methods 07/2005; 126(1-2):101-9. · 1.90 Impact Factor
Article: Effects of yellow fever vaccination.The Lancet 01/2002; 358(9296):1909. · 39.06 Impact Factor
Journal of Clinical Virology 36 (2006) 156–158
Yellow fever vaccine-associated viscerotropic
disease and death in Spain
A. Doblasa,1, C. Domingob,∗,1, H.G. Baec,1, C.L. Boh´ orquezd, F de Oryb,
M. Niedrigc, D. Moraa, F.J. Carrascoa, A. Tenoriob
aEmergency and Critical Care Department, Juan Ram´ on Jim´ enez Hospital, Huelva, Spain
bNational Center for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
cRobert Koch Institute, Berlin, Germany
dPathology Department, Juan Ram´ on Jim´ enez Hospital, Huelva, Spain
Received 15 November 2005; received in revised form 3 February 2006; accepted 5 February 2006
Yellow fever vaccine-associated viscerotropic disease (YEL-AVD) is a recently described severe adverse event after yellow fever
vaccination, and some cases have been reported in different countries [Anonymous. Effects of yellow fever and vaccination. Lancet
2001;358(9296):1907–9]. Herein we describe a YEL-AVD case in a young woman, who died after vaccination with 17D-204 strain. Clinical,
serological and immunochemical analysis as well as virus detection, quantification, sequence analysis and cytokine release, were performed.
Further investigations on yellow fever vaccine adverse events, and carefully analysis of the immune response elicited are important tasks for
© 2006 Elsevier B.V. All rights reserved.
Keywords: Yellow fever vaccine; Viscerotropic adverse event
On 14 October 2004, a 26-year-old Caucasian woman
from Huelva (Spain) received single doses of diphtheria, and
tetanus vaccine and was primary vaccinated against yellow
travel counselling. She had no pathologies, no known risk
Abbreviations: YF, yellow fever; YFV, yellow fever virus; YF-AVD,
yellow fever vaccine-associated viscerotropic disease; HIV, human immun-
odeficiency virus; RT-PCR, reverse transcription polimerase chain reaction;
IgM, immunoglobulin M; IgG, immunoglobulin G; GE, genome equiva-
lents; ml, millilitres; g, grammes; Nt Ab, neutralising antibodies; GRO;
GRO-?; IL-6, interlekin-6; IL-8, interlekin-8; IL-10, interlekin-10; MCP-1;
GCSF; GM-CSF; IL-1?, interleukin-1?; IL-13, interleukin-13; MIG; TGF-
?1, tumor growth factor ?1
∗Corresponding author at: Laboratory of Arboviruses and Imported Viral
Diseases, Diagnostic Microbiology Service, National Center for Microbiol-
ogy, Instituto de Salud Carlos III, Carretera de Majadahonda a Pozuelo km
2, Majadahonda 28220, Madrid, Spain. Tel.: +34 918 223 954;
fax: +34 915 097 919.
E-mail address: email@example.com (C. Domingo).
1These authors contributed equally to this work.
factors, and did not receive regular medication at the time of
Three days later, she developed pain and signs of inflam-
scribed. On 21 October she returned to the emergency room
tent fever. Chest and abdominal radiographies were normal.
Laboratory tests showed thrombocytopaenia and increased
levels of creatinine, aminotranferases, and bilirubin (Fig. 1).
The patient was admitted to the intensive care unit, where a
developed multiorgan failure and respiratory distress that
could not be controlled, and finally deteriorated to refractory
shock and death on 24 October 2004. Bacterial cultures from
samples of the patient’s blood, faeces and urine were nega-
A, B or C, influenza A or B, varicella-zoster, parainfluenza,
1386-6532/$ – see front matter © 2006 Elsevier B.V. All rights reserved.
A. Doblas et al. / Journal of Clinical Virology 36 (2006) 156–158
Fig. 1. Clinical presentation and analytical data. The figure presents clinical manifestations and biochemical data against the time course of YFV infection.
or respiratory sincytial viruses, nor by Leptospira inter-
rogans, Rickettsia conorii, Coxiella burnetti or Legionella
pneumophila. YF virus (YFV) growth was detected by RT-
PCR (S´ anchez-Seco et al., 2005) in VeroE6 cells 4 days after
inoculation with liver and kidney tissue homogenates, and
plasma. No virus could be cultured from serum.
microvesicular steatosis and hepatocellular necrosis affect-
ing lobular zone 2. Small haemorrhagic areas were observed
in the spleen, kidneys, heart and lungs. Vacuolar degenera-
tion of tubular epithelial cells in the kidneys, scarce areas
of muscle fibre vacuolation in the heart, and white pulp
depletion and histiocytic aggregates in the spleen were also
observed. Cytoplasmatic immunostaining of YFV-antigen
was observed in many hepatocytes and few cardiac muscle
fibre and renal tubular cells; no immunostaining was
observed in the spleen, lung, skin or gastrointestinal tract.
Serum obtained 8 days after vaccination showed specific
anti-YFV IgM, but no IgG, by indirect immunefluorescence
using YFV-infected cells, and a high titre (1:512) of neu-
tralising antibodies in a microneutralisation assay. RT-PCR
showed the presence of flavivirus RNA (S´ anchez-Seco et al.,
2005) in the liver, kidney, plasma and serum. No amplifica-
tion was detected in whole blood due to the presence of PCR
Virus load was determined by real-time PCR (Bae
et al., 2003). High amounts of YFV were detected
in the liver (6.2×109genome equivalents/g) and kid-
4×105GE/ml were detected in serum, plasma and whole
The complete consensus sequence (GenBank accession
number DQ118157) for the YFV present in liver and kidney
was compared to the sequence of the YFV 17D Stamaril®
vaccine strain. Only two silent nucleotide substitutions and
no base insertions or deletions were detected (Fig. 2).
CSF, IL-1?, IL-13, MIG and TGF-?1 showed weak signals,
production compartments of immune response.
The time elapsing from vaccination to onset of symptoms
association between YF vaccine and the clinical manifesta-
tions leading to death in this patient.
In contrast to typical wild-type YF, the patient showed
no bradycardia, haemorrhage was not prominent, hepatic
2001; Gerasimon and Lowry, 2005).
Fig. 2. Virological study. Results on the virological investigation in serum samples obtained 8 days after vaccination and in post-mortem tissues recovered 10
days after vaccination are tabulated.
A. Doblas et al. / Journal of Clinical Virology 36 (2006) 156–158
No adverse events associated with the same vaccine batch
have been reported by others. It is unclear whether other
factors such as host susceptibility may have led to the fatal
and a careful analysis of virological and immunological
markers are important tasks for the future.
The authors thank Dr. Yves Girerd-Chambaz (Sanofi-
(Stamaril®),Dr.F.J.Garc´ ıaPe˜ naforexcludingLeptospirasp.
infection, and Dr. M. Cabrerizo, F. Molero, and N. Reyes for
their helpful assistance in the laboratory work. The authors
are grateful to Dr. J.E. Mej´ ıa for assisting in manuscript
preparation. Dr. C. Domingo is supported by an agreement
between the Public Health Division of the Spanish Ministry
of Health (DGSP-MSC) and the Instituto de Salud Carlos
III (ISCIII) for the development of the Haemorrhagic Viral
Fevers Surveillance and Control Programme in Spain. The
collaboration between the National Centre for Microbiology
Network for Imported Viral Diseases (ENIVD).
Anonymous. Effects of yellow fever vaccination. Lancet 2001;358(9296):
Bae HG, Nitsche A, Teichmann A, Biel SS, Niedrig M. Detection of
yellow fever virus: a comparison of quantitative real-time PCR and
plaque assay. J Virol Meth 2003;110(2):185–91.
Gerasimon G, Lowry K. Rare case of fatal yellow fever vaccine
associated viscerotropic disease. Southern Med J 2005;98(6):653–
S´ anchez-Seco MP, Rosario D, Domingo C, et al. Generic RT-nested-PCR
for detection of flaviviruses using degenerated primers and internal
control followed by sequencing for specific identification. J Virol Meth