Article

Confocal reflectance theta line scanning microscope for imaging human skin in vivo

Institute of Optics, University of Rochester, Rochester, New York, United States
Optics Letters (Impact Factor: 3.18). 05/2006; 31(7):942-4. DOI: 10.1364/OL.31.000942
Source: PubMed

ABSTRACT A confocal reflectance theta line scanning microscope demonstrates imaging of nuclear and cellular detail in human epidermis in vivo. Experimentally measured line-spread functions determine the instrumental optical section thickness to be 1.7 +/- 0.1 microm and the lateral resolution to be 1.0 +/- 0.1 microm. Within human dermis (through full-thickness epidermis), the measured section thickness is 9.2 +/- 1.7 microm and the lateral resolution is 1.7 +/- 0.1 microm. An illumination line is scanned directly in the pupil of the objective lens, and the backscattered descanned light is detected with a linear array, such that the theta line scanner consists of only seven optical components.

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