Epstein-Barr virus origin of lytic replication mediates association of replicating episomes with promyelocytic leukaemia protein nuclear bodies and replication compartments

Department of Virology, Imperial College Faculty of Medicine, St Mary's Campus, London, UK.
Journal of General Virology (Impact Factor: 3.18). 06/2006; 87(Pt 5):1133-7. DOI: 10.1099/vir.0.81589-0
Source: PubMed


Epstein-Barr virus (EBV) establishes a latent persistence from which it can be reactivated to undergo lytic replication. Late lytic-cycle gene expression is linked to lytic DNA replication, as it is sensitive to the same inhibitors that block lytic replication, and it has recently been shown that the viral origin of lytic replication (ori lyt) is required in cis for late-gene expression. During the lytic cycle, the viral genome forms replication compartments, which are usually adjacent to promyelocytic leukaemia protein (PML) nuclear bodies. A tetracycline repressor DNA-binding domain-enhanced green fluorescent protein fusion was used to visualize replicating plasmids carrying a tetracycline operator sequence array. ori lyt mediated the production of plasmid replication compartments that were associated with PML nuclear bodies. Plasmids carrying ori lyt and EBV itself were visualized in the same cells and replicated in similar regions of the nucleus, further supporting the validity of the plasmids for studying late-gene regulation.

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Available from: Robert E White, Aug 17, 2015
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    • "The viral proteins needed for amplification of EBV DNA have been identified functionally (Chiu et al., 2007; Fixman et al., 1992). These EBV proteins and multiple cellular factors have been shown to localize to discrete sites in productively infected nuclei (Amon et al., 2006; Bell et al., 2000; Daikoku et al., 2005, 2006; Kudoh et al., 2009; Park et al., 2008). It has also been shown that EBV must transit from its use of its latent origin of DNA synthesis, oriP, to use two lytic origins, oriLyt L and R, to support its DNA amplification during a productive cycle (Hammerschmidt and Sugden, 1988). "
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