Article

Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release

European Neuroscience Institute Göttingen, Grisebachstrasse 5, 37077 Göttingen, Germany.
Science (Impact Factor: 31.48). 06/2006; 312(5776):1051-4. DOI: 10.1126/science.1126308
Source: PubMed

ABSTRACT The molecular organization of presynaptic active zones during calcium influx–triggered neurotransmitter release is the focus
of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular
synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins
seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity.

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