Lycopene prevents adriamycin-induced testicular toxicity in rats.

Department of Pharmacology and Toxicology, University of Firat, Elaziğ, Turkey.
Fertility and sterility (Impact Factor: 3.97). 05/2006; 85 Suppl 1:1216-22. DOI: 10.1016/j.fertnstert.2005.11.035
Source: PubMed

ABSTRACT To investigate a possible protective role of lycopene on adriamycin (ADR)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches.
Experimental study.
Firat University Medical School, Experimental Research Centre, Elazig, Turkey.
Twenty four Sprague Dawley rats (8-weeks old)
Adriamycin (10 mg kg(-1)) was intraperitoneally injected and lycopene (4 mg kg(-1)) was administered by gavage in corn oil.
Reproductive organ weights were evaluated along with epididymal sperm concentration, motility and morphology. Testicular histological findings, oxidative status and plasma testosterone levels were also determined.
Lycopene ameliorated ADR-induced reductions in both testes and epididymis weights. ADR decreased sperm motility, increased total abnormal sperm rates, but epididymal sperm concentration was not changed compared to control. A marked normalization was achieved in sperm motility and morphology in pretreatment with lycopene. Although testosterone level was decreased in ADR group compared to control, no changes were observed in pretreatment group. An increase in malondialdehyde and a decrease reduced glutathione concentrations were detected in alone ADR group compared to control. Pretreatment with lycopene restored significantly malondialdehyde and reduced glutathione concentrations. ADR caused severe degenerative changes in germinative cells, atrophy in the diameter size of seminiferous tubules and germinative cell thickness. However, ADR-induced histopathological alterations were effectively reverted by pretreatment with lycopene.
This study clearly indicates that ADR treatment markedly impaired testicular function and that pretreatment with lycopene might prevent this toxicity.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To clarify the modulatory effects of daily consumption of pomegranate extract (PE), olive oil (OO) and Nagilla sativa oil (NSO) on antioxidant activity, sperm quality and pituitary-testicular axis of adult male wistar rats. Thirty-two adult male Wistar rats were divided into four equal groups, eight rats each. Using rat gastric tubes, 1.0 mL distilled water, 1.0 mL PE, 0.4 mL NSO and 0.4 mL OO were orally administered daily for 6 weeks in the first, second, third and fourth groups, respectively. Reproductive organs, body weight, sperm criteria, testosterone, FSH, LH, inhibin-B, lipid peroxidation, and antioxidant enzyme activities were investigated. At the end of the study protocol, analyses occurred at the same time. Data were analysed by ANOVA test and P<0.05 was considered to be a significant value. In all studied groups, malondialdehyde level was significantly decreased accompanied with an increases in glutathione peroxidase and glutathione. Rats treated with PE showed an increase in catalase activities accompanied with an increase in sperm concentration which was also observed in NSO group. In PE treated group, sperm motility was also increased accompanied with decreased abnormal sperm rate. NSO, OO and PE treated groups shows an insignificant effect on testosterone, inhibin-B, FSH and LH in comparison with control group. These results show that administration of PE, NSO and OO could modify sperm characteristics and antioxidant activity of adult male wistar rats.
    Asian Pacific Journal of Tropical Biomedicine 07/2013; 3(7):563-8.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to examine the protective effects of fish omega-3 (n-3) fatty acids on acute doxorubicin (DOX)-induced testicular apoptosis and oxidative damage. 24 male rats were divided into three groups: control, DOX-treated and DOX+fish n-3 fatty acids. Fish n-3 fatty acids (400 mg kg(-1) ) were given for 30 days by intragastric gavage. The rats received a single intraperitoneal injection of DOX (30 mg kg(-1) ) and were sacrificed after 48 h. The DOX+fish n-3 fatty acids group showed a decrease in malondialdehyde levels and increased activities of superoxide dismutase and glutathione peroxidase in comparison with the DOX-treated group. Acute DOX treatment caused severe damage such as disorganisation and separation of germ cells. The fish n-3 fatty acids-pretreated rats showed an improved histological appearance in the DOX-treated group. Our data indicate a reduction in the activity of terminal deoxynucleotidyl transferase mediated dUTP nick end labelling; there was a rise in the expression of proliferating cell nuclear antigen in testis tissues of the DOX+fish n-3 fatty acids group compared with DOX-treated group. These data suggested that fish n-3 fatty acids pre-treatment may be beneficial for spermatogenesis following acute DOX-induced testicular damage by decreasing germ cell apoptosis and oxidative stress.
    Andrologia 10/2013; · 1.55 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Doxorubicin (Dox), an effective anticancer agent, can impair testicular function leading to infertility. The present study aimed to explore the protective effect of propolis extract on Dox-induced testicular injury. Rats were divided into four groups (n=10). Group I (normal control), group II received propolis extract (200 mgkg(-1); p.o.), for 3 weeks. Group III received 18 mgkg(-1) total cumulative dose of Dox i.p. Group IV received Dox and propolis extract. Serum and testicular samples were collected 48 h after the last treatment. In addition, the effects of propolis extract and Dox on the growth of solid Ehrlich carcinoma in mice were investigated. Dox reduced sperm count, markers of testicular function, steroidogenesis and gene expression of testicular 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD) and steroidogenic acute regulatory protein (StAR). In addition, it increased testicular oxidative stress, inflammatory and apoptotic markers. Morphometric and histopathologic studies supported the biochemical findings. Treatment with propolis extract prevented Dox-induced changes without reducing its antitumor activity. Besides, administration of propolis extract to normal rats increased serum testosterone level coupled by increased activities and gene expression of 3ß-HSD and 17ß-HSD. Propolis extract may protect the testis from Dox-induced toxicity without reducing its anticancer potential.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 03/2014; · 2.99 Impact Factor