FIG E1. Signal intensity of CD14 measurements and ‘‘no template controls.’’DRn values, which indicate the
magnitude of signal generated by the PCR reaction, are given for number of PCR cycles for 12 and 5
representative experiments, respectively.
FIG E2. Prevalences of atopic sensitization, hay fever, and asthma for farm (black bars) and reference
(gray bars) children.
FIG E3. The x-axis shows deciles of EPS load per square meter of mattress (n 5 322). The y-axis shows
geometric means (dots) of gene expression of TLR2, TLR4, and CD14, respectively, which were normalized by
dividing by gene expression of 18s rRNA. In addition, the linear fit of the association (solid line), along with the
corresponding 95% CI (dashed line), is displayed. All analyses were adjusted for age, sex, family history of
atopy, parental education, environmental tobacco smoking, maternal smoking during pregnancy, number
of older siblings, contact with pets ever, child’s current exposure to a farming lifestyle, child’s exposure to
farm animals, and predominant farm milk consumption of the child.