Activity of the essential oil from Chenopodium ambrosioides grown in Cuba against Leishmania amazonensis.
ABSTRACT Current therapy against leishmaniasis is unsatisfactory. Efficacious and safe new drugs are needed. In this study, we show the leishmanicidal effect of an essential oil from Chenopodium ambrosioides against Leishmania amazonensis.
The tested product had a potent inhibitory action against promastigote and amastigote forms, with 50% effective dose values of 3.7 and 4.6 microg/ml, respectively. The essential oil showed a moderate toxicity on macrophages from BALB/c mice. An optimal dose of 30 mg/kg/day was effective when administered during 15 days by intraperitoneal route to BALB/c mice infected experimentally.
These studies revealed a potential source for the discovery of novel drugs to combat the leishmaniasis based on the traditional medicine.
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ABSTRACT: Essential oils are complex volatile compounds, naturally synthesized by various parts of the plant during the secondary metabolism of plants. A wide range plants having the medicinal properties have been explored and used for the extraction of essential oils worldwide due to their antimicrobial properties against the bacterial, fungal and viral pathogens. The presence of a large number of alkaloids, phenols, terpenes derivatives compounds and other antimicrobial compounds makes the essential oils more précised in their mode action against the ample variety of pathogenic microorganisms. Thus, the essential oils could be used as better supplements or alternatives against the pathogenic microorganisms. The aim of this review article is to focus on the antimicrobial activities of essential oils secreted by medicinal plants and the mechanisms involved in the inhibition of these pathogenic microorganisms
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ABSTRACT: Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%), γ -elemene (14.25%), and trans- β -elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μ g·mL(-1)) and amastigotes (IC50, 1.92 μ g·mL(-1)) suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μ g·mL(-1)); however, there appeared to be no toxicity at 50 μ g·mL(-1). While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity.Evidence-based Complementary and Alternative Medicine 02/2013; 2013:279726. DOI:10.1155/2013/279726 · 1.88 Impact Factor
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ABSTRACT: Protozoa cause many diseases such as leishmaniasis, giardiasis, amebiasis, trichomoniasis and Chagas disease. Metronidazole is the most commonly used drug in antiprotozoal therapy, but metronidazole, as well as other antiprotozoal drugs, can have several secondary effects. Essential oils have been successfully used in the treatment of diseases caused by protozoa. This is a review covering such research from the last 10 years. The main essential oils from 42 plant species demonstrate activity against protozoa, and those from the Lamiaceae family were the most studied with 16 species showing antiprotozoal effects. Other plant families containing species that demonstrate therapeutic effects include Verbenaceae with seven species, Asteraceae with four, Piperaceae with three and Annonaceae with two; the others nine families each had one species. Thymol was the main component found in eight species, followed by eugenol and terpinen-4-ol, which were found in four species, and carvacrol and camphor, which were identified in three and two species, respectively.Journal of medicinal plant research 04/2012; 6(15):2901-2908. · 0.88 Impact Factor