Activity of the essential oil from Chenopodium ambrosioides grown in Cuba against Leishmania amazonensis.

Parasitology Department, Institute of Tropical Medicine Pedro Kourí, Havana, Cuba.
Chemotherapy (Impact Factor: 2.07). 02/2006; 52(3):130-6. DOI: 10.1159/000092858
Source: PubMed

ABSTRACT Current therapy against leishmaniasis is unsatisfactory. Efficacious and safe new drugs are needed. In this study, we show the leishmanicidal effect of an essential oil from Chenopodium ambrosioides against Leishmania amazonensis.
The tested product had a potent inhibitory action against promastigote and amastigote forms, with 50% effective dose values of 3.7 and 4.6 microg/ml, respectively. The essential oil showed a moderate toxicity on macrophages from BALB/c mice. An optimal dose of 30 mg/kg/day was effective when administered during 15 days by intraperitoneal route to BALB/c mice infected experimentally.
These studies revealed a potential source for the discovery of novel drugs to combat the leishmaniasis based on the traditional medicine.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Chenopodium ambrosioides is an aromatic herb used by native people to treat parasitic diseases. The aim of this work is to compare the in vitro anti-leishmanial activity of the essential oil (EO) from C. ambrosioides and its major components (ascaridole, carvacrol and caryophyllene oxide) and study their mechanism of action and activity against a panel of microorganism. Antileishmanial activity and cytotoxicity of the EO and major components was study. In addition, experiments to elucidate the mechanism of action were perform and activities against other microorganisms (bacteria, fungi and protozoa) were evaluate. All products were active against promastigote and amastigote forms of Leishmania. Ascaridole exhibited the better antileishmanial activity and the EO the highest selectivity index. The exploration of the mechanism suggests that the products cause a breakdown of mitochondrial membrane potential and a modification of redox indexes. Only EO showed antiprotozoal effect against Plasmodium falciparum and Trypanosoma brucei; while no activity against bacteria and fungi was observed. Our results demonstrate the potentialities of EO in cellular and molecular system, which could be consider in future studies to develop new antileishmanial drugs with a wide anti-parasitic spectrum.
    Experimental Parasitology 10/2013; · 2.15 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Essential oils are complex volatile compounds, naturally synthesized by various parts of the plant during the secondary metabolism of plants. A wide range plants having the medicinal properties have been explored and used for the extraction of essential oils worldwide due to their antimicrobial properties against the bacterial, fungal and viral pathogens. The presence of a large number of alkaloids, phenols, terpenes derivatives compounds and other antimicrobial compounds makes the essential oils more précised in their mode action against the ample variety of pathogenic microorganisms. Thus, the essential oils could be used as better supplements or alternatives against the pathogenic microorganisms. The aim of this review article is to focus on the antimicrobial activities of essential oils secreted by medicinal plants and the mechanisms involved in the inhibition of these pathogenic microorganisms
    Issues in Biological Sciences and Pharmaceutical Research. 01/2014; 2(1):1-7.
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this review, literature data on phytochemical and biological investigations on the genus Pluchea are compiled. Pluchea is a genus of flowering plants in the Asteraceae family and comprises ca. 80 species distributed mainly in Northern and Southern America, Africa, Asia, and Australia. Sesquiterpenoids and flavonoids are the main constituents of this genus. Compounds isolated from plants of the Pluchea genus display a variety of biological properties, viz., anticancer, antileishmanial, immunosuppressive, antioxidant, anti-acetylcholinesterase, antimicrobial, trypanocidal, hepatoprotective, cytotoxic, larvicidal, anti-ulcer, anti-inflammatory, and antinociceptive activities.
    Chemistry & Biodiversity 11/2013; 10(11):1944-71. · 1.81 Impact Factor