Activity of the Essential Oil from Chenopodium ambrosioides Grown in Cuba against Leishmania amazonensis

University of Havana, La Habana, La Habana, Cuba
Chemotherapy (Impact Factor: 1.29). 02/2006; 52(3):130-6. DOI: 10.1159/000092858
Source: PubMed


Current therapy against leishmaniasis is unsatisfactory. Efficacious and safe new drugs are needed. In this study, we show the leishmanicidal effect of an essential oil from Chenopodium ambrosioides against Leishmania amazonensis.
The tested product had a potent inhibitory action against promastigote and amastigote forms, with 50% effective dose values of 3.7 and 4.6 microg/ml, respectively. The essential oil showed a moderate toxicity on macrophages from BALB/c mice. An optimal dose of 30 mg/kg/day was effective when administered during 15 days by intraperitoneal route to BALB/c mice infected experimentally.
These studies revealed a potential source for the discovery of novel drugs to combat the leishmaniasis based on the traditional medicine.

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    • "C. ambrosioides also exhibited in vitro leishmanicidal effects against L. amazonensis promastigotes [41]. In the same way, Monzote et al. [42] showed that an essential oil from C. ambrosioides inhibits the progression of leishmanial infection both in vitro and in vivo. The essential oil had a minimal inhibitoryconcentration and EC50 values of 27.82 and 3.74 μg/mL, respectively, against promastigotes of L. amazonensis [43]. "
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    ABSTRACT: This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of Alagoas, Brazil. Data were collected in the cities of Colonia Leopoldina, Novo Lino, and União dos Palmares, Alagoas state, from patients with cutaneous leishmaniasis (Leishmania amazonensis) who use medicinal plants to treat this disease. Plants extracts were tested at a concentration of 1-100 μg/mL in all experiments, except in an assay to evaluate activity against amastigotes, when 10 μg/mL was used. All plants extracts did not show deleterious activity to the host cell evidenced by LDH assay at 100, 10, and 1 μg/mL after 48 h of incubation. The plants extracts Hyptis pectinata (L.) Poit, Aloe vera L., Ruta graveolens L., Pfaffia glomerata (Spreng.) Pedersen, and Chenopodium ambrosioides L. exhibited direct activity against extracellular forms at 100 μg/mL; these extracts inhibited growth by 81.9%, 82.9%, 74.4%, 88.7%, and 87.4%, respectively, when compared with promastigotes. The plants extracts H. pectinata, A. vera, and R. graveolens also significantly diminished the number of amastigotes at 10 μg/mL, inhibiting growth by 85.0%, 40.4%, 94.2%, and 97.4%, respectively, when compared with control. Based on these data, we conclude that the five plants exhibited considerable leishmanicidal activity.
    Evidence-based Complementary and Alternative Medicine 07/2014; 2014(24):478290. DOI:10.1155/2014/478290 · 1.88 Impact Factor
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    • "The extraction products may vary in quality, quantity and in composition according to climate, soil composition, plant organ, age and vegetative cycle stage (Masotti et al., 2003; Angioni et al., 2006). Essential oils or some of their constituents are indeed effective against a large variety of organisms including bacteria and viruses (Duschatzky et al., 2005), fungi (Hammer et al., 2002) and protozoa (Monzote et al., 2006). The essential oils are known for their bactericidal, virucidal, fungicidal activity due to their medicinal properties against the wide range of pathogenic microorganisms. "
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    ABSTRACT: Essential oils are complex volatile compounds, naturally synthesized by various parts of the plant during the secondary metabolism of plants. A wide range plants having the medicinal properties have been explored and used for the extraction of essential oils worldwide due to their antimicrobial properties against the bacterial, fungal and viral pathogens. The presence of a large number of alkaloids, phenols, terpenes derivatives compounds and other antimicrobial compounds makes the essential oils more précised in their mode action against the ample variety of pathogenic microorganisms. Thus, the essential oils could be used as better supplements or alternatives against the pathogenic microorganisms. The aim of this review article is to focus on the antimicrobial activities of essential oils secreted by medicinal plants and the mechanisms involved in the inhibition of these pathogenic microorganisms
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    • "among these are Croton cajucara [6], Ocimum gratissimum [7], Copaifera cearensis [8], Chenopodium ambrosioides [9], Cymbopogon citratus [10] [11], and Lippia sidoides [12]. These studies have shown that essential oils can be a promising source of new drugs with anti-Leishmania activity. "
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    ABSTRACT: Eugenia uniflora L. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition of Eugenia uniflora L. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmania activity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%), γ -elemene (14.25%), and trans- β -elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μ g·mL(-1)) and amastigotes (IC50, 1.92 μ g·mL(-1)) suggested significant anti-Leishmania activity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μ g·mL(-1)); however, there appeared to be no toxicity at 50 μ g·mL(-1). While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmania activity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determine in vivo activity as well as additional mechanisms of the anti-Leishmania activity.
    Evidence-based Complementary and Alternative Medicine 02/2013; 2013(6):279726. DOI:10.1155/2013/279726 · 1.88 Impact Factor
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