Article

Task-based imaging of colon cancer in the Apc(Min/+) mouse model

The University of Arizona, Tucson, Arizona, United States
Applied Optics (Impact Factor: 1.78). 06/2006; 45(13):3049-62. DOI: 10.1364/AO.45.003049
Source: PubMed

ABSTRACT Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

Download full-text

Full-text

Available from: Urs Utzinger, Aug 23, 2015
0 Followers
 · 
136 Views
  • Source
    • "However, a fasting time of about 6 hours is suggested as an effective way to ensure uniformity in some studies such as positron emission tomography with F-FGD (Hildebrandt et al. 2008). In addition, when optical imaging is performed, it is necessary to consider dietary composition because food components such as chlorophyll can be a source of background autofluorescence (McNally et al. 2006). Mice should always have free access to water, even shortly before general anesthesia. "
    ILAR journal / National Research Council, Institute of Laboratory Animal Resources 09/2012; ILAR Journal(54). · 1.05 Impact Factor
  • Source
    • "This miniaturized 2 mm diameter catheter-based dual-modality system has been used to monitor the disease progression in mouse colon longitudinally, and is able to identify colorectal adenomas in murine models (Hariri et al., 2006; McNally et al., 2006; Hariri et al., 2007). In one study, McNally et al. used the OCT-LIF system to study healthy and adenomatous mouse colon. "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this chapter, we introduce several multimodal optical imaging techniques which combines OCT and fluorescence imaging. OCT provides high-resolution, cross-sectional imaging of tissue microstructure, while fluorescence imaging reveals the biochemical and/or molecular information. Multimodal optical imaging techniques which combine OCT and fluorescence imaging could provide morphological, molecular and functional information simultaneously, and have great potential in disease diagnostics and therapy. Several such multimodal systems as well as their biomedical applications are introduced and discussed in this chapter. An ongoing development of novel multimodal system (OCT/FLOT) is also briefly introduced.
    Advances in Lasers and Electro Optics, 04/2010; , ISBN: 978-953-307-088-9
  • [Show abstract] [Hide abstract]
    ABSTRACT: Ovarian cancer is the fifth leading cause of cancer death in women, in part because of the limited knowledge about early stage disease. We develop a novel rat model of ovarian cancer and perform a pilot study to examine the harvested ovaries with complementary optical imaging modalities. Rats are exposed to repeated daily dosing (20 days) with 4-vinylcyclohexene diepoxide (VCD) to cause early ovarian failure (model for postmenopause), and ovaries are directly exposed to 7,12-dimethylbenz(a)anthracene (DMBA) to cause abnormal ovarian proliferation and neoplasia. Harvested ovaries are examined with optical coherence tomography (OCT) and light-induced fluorescence (LIF) at one, three, and five months post-DMBA treatment. VCD causes complete ovarian follicle depletion within 8 months after onset of dosing. DMBA induces abnormal size, cysts, and neoplastic changes. OCT successfully visualizes normal and abnormal structures (e.g., cysts, bursa, follicular remnant degeneration) and the LIF spectra show statistically significant changes in the ratio of average emission intensity at 390:450 nm between VCD-treated ovaries and both normal cycling and neoplastic DMBA-treated ovaries. Overall, this pilot study demonstrates the feasibility of both the novel animal model for ovarian cancer and the ability of optical imaging techniques to visualize ovarian function and health.
    Journal of Biomedical Optics 01/2006; 11(4):041123. DOI:10.1117/1.2236298 · 2.75 Impact Factor
Show more