Task-based imaging of colon cancer in the Apc(Min/+) mouse model

The University of Arizona, Tucson, Arizona, United States
Applied Optics (Impact Factor: 1.78). 06/2006; 45(13):3049-62. DOI: 10.1364/AO.45.003049
Source: PubMed

ABSTRACT Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

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    • "However, a fasting time of about 6 hours is suggested as an effective way to ensure uniformity in some studies such as positron emission tomography with F-FGD (Hildebrandt et al. 2008). In addition, when optical imaging is performed, it is necessary to consider dietary composition because food components such as chlorophyll can be a source of background autofluorescence (McNally et al. 2006). Mice should always have free access to water, even shortly before general anesthesia. "
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    • "This miniaturized 2 mm diameter catheter-based dual-modality system has been used to monitor the disease progression in mouse colon longitudinally, and is able to identify colorectal adenomas in murine models (Hariri et al., 2006; McNally et al., 2006; Hariri et al., 2007). In one study, McNally et al. used the OCT-LIF system to study healthy and adenomatous mouse colon. "
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