Article

Distinct activities of Msx1 and Msx3 in dorsal neural tube development.

Center for Basic Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
Development (impact factor: 6.6). 04/2004; 131(5):1017-28. DOI:10.1242/dev.00994 pp.1017-28
Source: PubMed

ABSTRACT Patterning of the dorsal neural tube involves Bmp signaling, which results in activation of multiple pathways leading to the formation of neural crest, roof plate and dorsal interneuron cell types. We show that constitutive activation of Bmp signaling at early stages (HH10-12) of chick neural tube development induces roof-plate cell fate, accompanied by an increase of programmed cell death and a repression of neuronal differentiation. These activities are mimicked by the overexpression of the homeodomain transcription factor Msx1, a factor known to be induced by Bmp signaling. By contrast, the closely related factor, Msx3, does not have these activities. At later stages of neural tube development (HH14-16), dorsal progenitor cells lose their competence to generate roof-plate cells in response to Bmp signaling and instead generate dorsal interneurons. This aspect of Bmp signaling is phenocopied by the overexpression of Msx3 but not Msx1. Taken together, these results suggest that these two different Msx family members can mediate distinct aspects of Bmp signaling during neural tube development.

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Keywords

Bmp signaling
 
cell death
 
chick neural tube development induces roof-plate cell fate
 
constitutive activation
 
distinct aspects
 
dorsal interneuron cell types
 
dorsal interneurons
 
dorsal neural tube
 
dorsal progenitor cells
 
homeodomain transcription factor Msx1
 
Msx1
 
Msx3
 
multiple pathways
 
neural
 
neural tube development
 
neuronal differentiation
 
Patterning
 
roof plate
 
roof-plate cells
 
two different Msx family members
 

Ying Liu