Lam KS Discovery of novel metabolites from marine actinomycetes. Curr. Opin. Microbiol. 9, 245-251

Department of Microbiology and Anti-infective Discovery, Nereus Pharmaceuticals, Inc., 10480 Wateridge Circle, San Diego, CA 92121, USA.
Current Opinion in Microbiology (Impact Factor: 5.9). 07/2006; 9(3):245-51. DOI: 10.1016/j.mib.2006.03.004
Source: PubMed


Recent findings from culture-dependent and culture-independent methods have demonstrated that indigenous marine actinomycetes exist in the oceans and are widely distributed in different marine ecosystems. There is tremendous diversity and novelty among the marine actinomycetes present in marine environments. Progress has been made to isolate novel actinomycetes from samples collected at different marine environments and habitats. These marine actinomycetes produce different types of new secondary metabolites. Many of these metabolites possess biological activities and have the potential to be developed as therapeutic agents. Marine actinomycetes are a prolific but underexploited source for the discovery of novel secondary metabolites.

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    • "Several actinomycetes, found to be proficient to produce antimicrobial compounds and halotolerant enzymes, have been reported from the coastal solar salterns (Vasavada et al. 2006; Aruljose et al. 2011). Actinomycetes are the most economically and biotechnologically valuable prokaryotes (Lam 2006) On the other hand, a great metabolic diversity and biotechnological potential has been found in halophilic and halotolerant microorganisms. "
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    ABSTRACT: Antagonistic Streptomyces spp. AJ8 was isolated and identified from the Kovalam solar salt works in India. The anti-microbial NRPS cluster gene was characterized by PCR, sequencing and predict the secondary structure analysis. The secondary metabolites will be extracted from different organic solvent extraction and studied the antibacterial, anti-fungal, antiviral and anticancer activities. In vitro antagonistic activity results revealed that, Streptomyces spp. AJ8 was highly antagonistic against Staphylococcus aureus, Aeromonas hydrophila WPD1 and Candida albicans. The genomic level identification revealed that, the strain was confirmed as Streptomyces spp. AJ8 and submitted the NCBI database (KC603899). The NRPS gene was generated a single gene fragment of 781 bp length (KR491940) and the database analysis revealed that, the closely related to Streptomyces spp. SAUK6068 and S. coeruleoprunus NBRC15400. The secondary metabolites extracted with ethyl acetate was effectively inhibited the bacterial and fungal growth at the ranged between 7 and 19.2 mm of zone of inhibition. The antiviral activity results revealed that, the metabolite was significantly (P < 0.001) controlled the killer shrimp virus white spot syndrome virus at the level of 85 %. The metabo-lite also suppressed the L929 fibroblast cancer cells at 35.7 % viability in 1000 µg treatment.
    AMB Express 12/2015; 5(1). DOI:10.1186/s13568-015-0143-2
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    • "Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests 3 cellulase, amylase, chitinase, gelatinase, caseinase and lipase (Bredholt et al., 2008). Also they are considered as a good and important source to identify and discover new biological products so it is important to find new actinomycetes in new habitats such as mangrove forests (Lam, 2006; Malarvizhi, 2006). Amylase is one of the most important industrial enzymes and each year several tons of it are used in many industrial fields in Iran (Moghbeli and Noshiri, 2009). "
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    ABSTRACT: In this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples were diluted then they were purified on the starch (casein agar) culture and Woodruff. After that they were examined in terms of amylase production on agar–starch culture. The activity of the produced amylase by the isolated aquatic actinomycetes was measured by dinitrosalicylic acid (DNS) method. The results showed that aquatic actinomycetes were isolated from 86 per 100 places in spring (86%) and from 61 per 100 places in summer (61%). The highest rates of producing enzyme were related to isolated samples in spring (62.97 U/ml). Biochemical and Bergey’s book tests showed that the most isolated aquatic actinomycetes belonged to Streptomyces genus. As regards this, it is economical and easy to isolate the aquatic actinomycetes which produce amylase that is used in different industries in Iran from the sediments of mangrove forests of the south of Iran. So the isolated strains in this study can be suitable candidates for amylase production after genetic manipulation.
    Egyptian Journal of Aquatic Research 05/2015; 15(2). DOI:10.1016/j.ejar.2015.04.003
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    • "Endophytic actinomycetes, recovered from healthy surface sterilized tissues in particular are considered as potential source for the production of secondary metabolites, various natural products with antimicrobial, antioxidants and plant growth promoting activities (Merckx et al., 1987; Lam, 2006; Nimnoi et al., 2010). There is increasing evidences for the existence of new endophytic actinomycetes within various tissues of medicinal plants, and some produce bioactive compounds with novel chemical structures (Qin et al., 2008; Nimnoi et al., 2010). "
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    ABSTRACT: Microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. To date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. In this study, 42 endophytic actinomycetes were isolated from different organs of seven selected medicinal plants. The highest number of isolates (n=22, 52.3%) of actinomycetes was isolated from roots, followed by stems (n=9, 21.4%), leaves (n=6, 14.2%), flowers (n=3, 7.1%) and petioles (n=2, 4.7%). The genus Streptomyces was the most dominant among the isolates (66.6%) in both the locations (Dampa TRF and Phawngpuii NP, Mizoram, India). From a total of 42 isolates, 22 isolates were selected for further studies based on their ability to inhibit one of the tested human bacterial or fungal pathogen. Selected isolates were identified based on 16S rRNA gene analysis and subsequently the isolates were grouped to four different genera; Streptomyces, Brevibacterium, Microbacterium and Leifsonia. Antibiotic sensitivity assay was performed to understand the responsible antimicrobials present in the isolates showing the antimicrobial activities and revealed that the isolates were mostly resistant to penicillin G and ampicillin. Further, antimicrobial properties and antibiotic sensitivity assay in combination with the results of amplification of biosynthetic genes polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) showed that the endophytic actinomycetes associated with the selected medicinal plants have broad-spectrum antimicrobial activity. This is the first report of the isolation of Brevibacterium sp., Microbacterium sp. and Leifsonia xyli from endophytic environments of medicinal plants, Mirabilis jalapa and Clerodendrum colebrookianum. Our results emphasize that endophytic actinomycetes associated with medicinal plants are an unexplored resource for the discovery of biologically active compounds.
    Frontiers in Microbiology 03/2015; DOI:10.3389/fmicb.2015.00273 · 3.99 Impact Factor
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