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Lam KS Discovery of novel metabolites from marine actinomycetes. Curr. Opin. Microbiol. 9, 245-251

Department of Microbiology and Anti-infective Discovery, Nereus Pharmaceuticals, Inc., 10480 Wateridge Circle, San Diego, CA 92121, USA.
Current Opinion in Microbiology (Impact Factor: 7.22). 07/2006; 9(3):245-51. DOI: 10.1016/j.mib.2006.03.004
Source: PubMed

ABSTRACT Recent findings from culture-dependent and culture-independent methods have demonstrated that indigenous marine actinomycetes exist in the oceans and are widely distributed in different marine ecosystems. There is tremendous diversity and novelty among the marine actinomycetes present in marine environments. Progress has been made to isolate novel actinomycetes from samples collected at different marine environments and habitats. These marine actinomycetes produce different types of new secondary metabolites. Many of these metabolites possess biological activities and have the potential to be developed as therapeutic agents. Marine actinomycetes are a prolific but underexploited source for the discovery of novel secondary metabolites.

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    • "Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests 3 cellulase, amylase, chitinase, gelatinase, caseinase and lipase (Bredholt et al., 2008). Also they are considered as a good and important source to identify and discover new biological products so it is important to find new actinomycetes in new habitats such as mangrove forests (Lam, 2006; Malarvizhi, 2006). Amylase is one of the most important industrial enzymes and each year several tons of it are used in many industrial fields in Iran (Moghbeli and Noshiri, 2009). "
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    ABSTRACT: In this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples were diluted then they were purified on the starch (casein agar) culture and Woodruff. After that they were examined in terms of amylase production on agar–starch culture. The activity of the produced amylase by the isolated aquatic actinomycetes was measured by dinitrosalicylic acid (DNS) method. The results showed that aquatic actinomycetes were isolated from 86 per 100 places in spring (86%) and from 61 per 100 places in summer (61%). The highest rates of producing enzyme were related to isolated samples in spring (62.97 U/ml). Biochemical and Bergey’s book tests showed that the most isolated aquatic actinomycetes belonged to Streptomyces genus. As regards this, it is economical and easy to isolate the aquatic actinomycetes which produce amylase that is used in different industries in Iran from the sediments of mangrove forests of the south of Iran. So the isolated strains in this study can be suitable candidates for amylase production after genetic manipulation.
    05/2015; 15(2). DOI:10.1016/j.ejar.2015.04.003
    • "To date, actinomycetes have been isolated primarily from soil. However, recently, the rate of novel compound discovery from the widely explored terrestrial strains has decreased significantly (Lam 2006). To overcome this problem, actinomycetes have been isolated from other resources such as a desert ecosystem and marine sediments (Bull et al. 2005; Bull and Stach 2007). "
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    ABSTRACT: The present study was attempted to determine the antimicrobial potential of actinomycetes from the Great Indian Thar Desert. A total of 100 different morphotypes based on phenotypic characterization were isolated from desert ecosystems located in the northwest of India and tested for their antimicrobial activity by the cross-streak method. Among the strains tested, 13 actinomycetes exhibiting strong antimicrobial activities against several test organisms, including multidrug resistant bacteria (MRSA) were chosen for a phylogenetic diversity study. The results of 16S rRNA gene sequencing showed their affiliation to actinobacterial genera: Actinomadura, Nocardia, Nonomuraea, Spirillispora, and Streptomyces. Three of these isolates were considered to be new members of the Streptomyces genus and another strain also seemed to be a new species of the genus Spirillispora. Among these strains, five were chosen to study the bioactive products using Q-Tof-MS because of their broad spectrum activity against the panel of test pathogens used. The results showed that they produce many known compounds and might produce few unknown compounds as well. This is the first such report on the selective isolation of actinomycetes from the Great Indian Thar desert, and their screening for antibacterial potential. This ecosystem has never before been explored to this extent.
    Annals of Microbiology 01/2015; DOI:10.1007/s13213-014-1028-3 · 1.04 Impact Factor
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    • "Actinomycetes are Gram positive bacteria and an important source of antibiotics (Valli et al., 2012). They have high G+C content 55-77% (Lo et al., 2002; Ningthoujam et al., 2009; Lam et al., 2006 and Ndonde et al., 2000). They belong to order actinomycetales and composed of around eighty genera (Stackebrandt et al., 1997 and Goodfellow et al., 1983) .They are distributed ubiquitously including water, soil, and marine (Gebreyohannes et al., 2013). "
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    ABSTRACT: Actinomycetes are abundantly present in soil and they produce a variety of antimicrobial compounds that can be used as chemotherapeutic agent in order to limit the infection. In present study, actinomycetes were isolated from different ground soils. Primarily these isolates were screened for extracellular metabolites production by conventional methods. Cross streak method and double agar overlay methods were used in this screening. Initially we have isolated 33 actinomycetes strains from different soil samples and screened them for antimicrobial potential. About 51.51 % of isolated strains showed the antagonistic properties against one or two tested gram positive bacteria. The best strain IAS 1, IAS 7, IAS 10, IAS 11, showed maximum zone of inhibition against M.luteus. The chemical nature of ISA 10 was assessed by simply heating the supernatant and we found that the extra cellular metabolite activity was absent in heated sample suggesting the protein nature of it
    International Journal of Current Research 01/2015; 7(1):12078-12083.
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