[Scoring criteria of aneuploidy frequency in interphase nuclei by fish-analysis].

Abstract

Principles and approaches of assessment of numerical chromosome abnormalities in interphase nuclei by fluorescent in situ hybridization technology are reviewed. The authors' own scoring criteria of results of hybridization of centromere-specific DNA-probes with chromosomal targets in somatic cells by dual color FISH-analysis are suggested. Use of these scoring criteria allows to reduce the level of artificial hypoploidy, whose its frequency becomes similar to that of hyperploidy. This fact indicates that chromosomal nondisjunction is a major mechanism of aneuploidy induction, rather than of chromosome loss.