Propolis extracts exhibit an immunoregulatory activity in an OVA-sensitized airway inflammatory animal model
ABSTRACT Propolis, which has been used widely in folk medicine, has been shown to exhibit various biological activities but its immunoregulatory and anti-inflammatory activities in intact animals have not been well studied. We investigated these activities of propolis using an ovalbumin-induced asthma animal model. Mice were immunized and sensitized by exposure to ovalbumin (OVA) antigen and administered with low- (65 mg/kg body weight) and high-dose (325 mg/kg body weight) propolis water extracts by tube feeding. The serum OVA-specific IgE titer and cytokine profiles in cultured splenocytes and bronchoalveolar lavage fluids (BALF) were analyzed. The number of eosinophils in BALF was counted. Here we demonstrate that propolis extracts can suppress the serum levels of OVA-specific IgE and IgG(1), and airway hyperresponsiveness (AHR) in OVA-sensitized mice. There are no significant differences in the concentration of eotaxin or the number of eosinophils in BALF among the four groups. However, the higher dose of propolis extracts decreases the level of IL-5 in BALF. The splenocytes from mice administered with propolis extracts (low- and high-dose groups) exhibit a strong inhibition of IL-10 secretion and up-regulation of IFN-gamma secretion in splenocytes stimulated with concanavalin A (ConA). In addition, cytokine (IFN-gamma, IL-6, and IL-10) secretion in OVA-stimulated splenocytes from the propolis groups was significantly lower than that in the control group. These results suggest that propolis extracts may be a potential novel therapeutic agent for asthma.
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ABSTRACT: This chapter expresses the effects of propolis on oxidative stress in animals. The term “stress” was first coined by the endocrinologist Hans Selye (1936) more than 70 years ago to define the physiological adaptive responses of the organism to emotional or physical threats (stressors), whether real or perceived (Selye, 1936). Factors causing stress include physiological factors, such as climate, environment, nutrition, and diseases, and physical conditions, such as cage density and transport. Under stress, rapid and temporary changes occur in the body initially; with continuous stress, these are followed by permanent and irreversible changes (Tatli Seven, 2008). Stress responses are characterized as primary, secondary and tertiary. The primary stress response is a neuroendocrine response leading to corticosteroid and catecholamine release. The secondary stress response includes changes in plasma and tissue ion and metabolite levels induced by neuroendocrine hormones. The changes in disease resistance, growth, condition factor, and behaviors at a whole organism level are tertiary responses (Wedemeyer et al., 1990). Finally, a decline in yield and resistance to diseases may occur. Animals under stress become ill more easily, and excess medicine may be necessary to maintain health. As a result, drug residues increase in animal products and threaten public health directly. Stock health and welfare management are key factors in animal health and food safety. For this reason, stress conditions in animals need to be examined carefully (Tatli Seven, 2008). Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. During times of environmental stress (e.g. ultraviolet or heat exposure, environmental pollutant), ROS levels can increase dramatically.This may result in significant damage to cell structures.This cumulates into a situation known as oxidative stress. This chapter was written to demonstrate the importance of propolis that have effects antioxidant, antibacterial, antitumor, anti-inflammatory etc. in animals under oxidative stress.OXIDATIVE STRESS – ENVIRONMENTAL INDUCTION AND DIETARY ANTIOXIDANTS, First Edition edited by Volodymyr I. Lushchak, 04/2012: chapter The Effects of Propolis in Animals Exposed Oxidative Stress: pages 267-288; InTech., ISBN: 978-953-51-0553-4
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ABSTRACT: INTRODUCTION: Propolis has plenty of biological and pharmacological properties and its mechanisms of action have been widely investigated in the last years, using different experimental models in vitro and in vivo. Researchers have been interested in the investigation of isolated compounds responsible for propolis action; however, there is lack of clinical research on the effects of propolis. STRATEGY AND OBJECTIVES: Since propolis-containing products have been marketed and humans have used propolis for different purposes, the goal of this review is to discuss the potential of propolis for the development of new drugs, by comparing data from the literature that suggest candidate areas for the establishment of drugs against tumors, infections, allergy, diabetes, ulcers and with immunomodulatory action. CONCLUSIONS: The efficacy of propolis in different protocols in vitro and in vivo suggests its therapeutic properties, but before establishing a strategy using this bee product, it is necessary to study: (a) the chemical nature of the propolis sample. (b) Propolis efficacy should be compared to well-established parameters, e.g. positive or negative controls in the experiments. Moreover, possible interactions between propolis and other medicines should be investigated in humans as well. (c) Clinical investigation is needed to evaluate propolis potential in patients or healthy individuals, to understand under which conditions propolis may promote health. Data point out the importance of this research field not only for the readers and researchers in the scientific community waiting for further clarification on the potential of propolis but also for the pharmaceutical industry that looks for new drugs.Journal of ethnopharmacology 10/2010; 133(2):253-60. DOI:10.1016/j.jep.2010.10.032 · 2.94 Impact Factor
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DESCRIPTION: Pinar Tatli Seven, Seval Yilmaz, Ismail Seven and Gulizar Tuna Kelestemur