Article

Development of an analytical method for the determination of anthracyclines in hospital effluents.

Department of Medicine I, Clinical Division of Oncology, Medical University of Vienna, Waehringer Guertel 18-20, Vienna, Austria.
Chemosphere (impact factor: 3.21). 11/2006; 65(8):1419-25. DOI:10.1016/j.chemosphere.2006.03.069 pp.1419-25
Source: PubMed

ABSTRACT Little is known about the fate of cytostatics after their elimination from humans into the environment. Being often very toxic compounds, their quantification in hospital effluents may be necessary to individualise the putative magnitude of pollution problems. We therefore developed a method for the determination of the very important group of anthracyclines (doxorubicin, epirubicin, and daunorubicin) in hospital effluents. Waste water samples were enriched by solid phase extraction (concentration factor 100), analysed by reversed-phase high performance liquid chromatography (RP-HPLC), and monitored by fluorescence detection. This method is reproducible and accurate within a range of 0.1-5 micro g l(-1) for all compounds (limits of quantification: 0.26-0.29 micro g l(-1) ; recoveries >80%). The applicability of the method was proven by chemical analysis of hospital sewage samples (range: 0.1-1.4 micro g l(-1) epirubicin and 0.1-0.5 micro g l(-1) doxorubicin). Obtained over a time period of one month, the results were in line with those calculated by an input-output model. These investigations show that the examined cytostatics are easily detectable and that the presented method is suitable to estimate the dimension of pharmaceutical contamination originating from hospital effluents.

0 0
 · 
0 Bookmarks
 · 
40 Views
  • Source
    Article: Genotoxic effects of wastewater from an oncological ward.
    Franziska Ferk, Miroslav Misík, Tamara Grummt, Bernhard Majer, Maria Fuerhacker, Christoph Buchmann, Marius Vital, Maria Uhl, Katharina Lenz, Britta Grillitsch, Wolfram Parzefall, Armen Nersesyan, Siegfried Knasmüller
    [show abstract] [hide abstract]
    ABSTRACT: Aim of this study was the evaluation of the genotoxic activities of hospital wastewaters. Samples from an oncological ward of the general hospital of Vienna, Austria, were tested in the Salmonella/microsome assay in strains TA98, TA100 and TA1535 with or without metabolic activation, and in the single-cell gel electrophoresis (SCGE) assay with primary rat hepatocytes. In the bacterial tests, consistently negative results were obtained while in the experiments with liver cells a significant and dose-dependent induction of DNA damage (up to two-fold over the background) was found. Membrane filtration resulted in a substantial (62-77%) reduction of these effects, while additional treatments (activated carbon filtration and UV-irradiation) did not lead to a further decrease of the genotoxic activity of the samples. SCGE experiments with cisplatin, carboplatin and 5-fluorouracil, which were detected in the water samples, showed that these cytostatics cause a significant induction of DNA damage only at concentrations that are substantially higher than those in the native waters. These findings indicate that other chemicals, possibly quaternary ammonium compounds, account for the effects of the hospital wastewaters.
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 12/2008; 672(2):69-75. · 2.85 Impact Factor
  • Source
    Article: Addressing safety issues of health care personnel, family members, and the environment.
    Jessica Livneh, Kent Waddington, Jair Bar
    Journal of Clinical Oncology 02/2010; 28(13):e209; author reply e210. · 18.37 Impact Factor
  • Source
    Article: Analysis of anticancer drugs in sewage water by selective SPE and UPLC-ESI-MS-MS.
    Jie Yin, Yi Yang, Keji Li, Jing Zhang, Bing Shao
    [show abstract] [hide abstract]
    ABSTRACT: A trace analytical procedure was developed to assay the anticancer drugs methotrexate, azathioprine, doxorubicin, doxorubicinol, vincristine, ifosfamide, cyclophosphamide, etoposide, and procarbazine in water samples from sewage treatment plants. After concentration and purification using Oasis HLB solid-phase extraction cartridges and Oasis WAX cartridges, the analytes were separated using ultra-high performance liquid chromatography coupled with the electrospray ionization tandem mass spectrometry operating in the positive ion mode. The method showed good precision and accuracy. Recoveries of all analytes were in the range of 45.3-108.9% with relative standard deviations between 2.4-24.5%. The limits of detection for influent and effluent sewage water were in the range of 0.6-7.0 ng/L and 0.5-3.5 ng/L, respectively. It is expected that this method will be applied to investigate the environmental occurrence of anticancer drugs in sewage water.
    Journal of chromatographic science 01/2010; 48(10):781-9. · 0.88 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

applicability
 
chemical analysis
 
concentration factor 100
 
epirubicin
 
examined cytostatics
 
fluorescence detection
 
hospital effluents
 
hospital sewage samples
 
individualise
 
input-output model
 
limits
 
Obtained
 
pharmaceutical contamination originating
 
pollution problems
 
putative magnitude
 
quantification
 
reversed-phase
 
solid phase extraction
 
time period
 
Waste water samples