Article

[Identification of biomarkers in the serum of the patients with poorly differentiated gastric cancer].

Department of Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery 06/2006; 9(3):253-6. pp.253-6
Source: PubMed

ABSTRACT To investigate the application of proteomics in the field of serology,and to screen the differential expression proteins related with poorly differentiated gastric carcinoma.
Two-dimensional electrophoresis (2-DE) was applied to segregate the total proteins in the serum form gastric cancer patients and health volunteers. After staining,the differential expression proteins were analyzed using PDQuest software,and identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).
Electrophoresis figures with high resolution and reproducibility were obtained. Six differential expression proteins were found only in the serum from gastric cancer patients, while four other proteins from healthy volunteers.
Protein expression is differential in the serum from the gastric cancer patients and health volunteers. It is hopeful to find the biomarkers related with poorly differentiated gastric carcinoma using proteomics.

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Keywords

2-DE
 
differential expression proteins
 
flight mass spectrometry
 
gastric cancer patients
 
MALDI-TOF-MS
 
matrix-assisted laser desorption/ionization-time
 
poorly differentiated gastric carcinoma
 
Protein expression
 
proteins
 
proteomics
 
serum form gastric cancer patients
 
total proteins
 
Two-dimensional electrophoresis