From a morphologic and functional point of view the thyroid can be considered as both an exocrine and endocrine organ. Firstly, thyroglobulin is secreted at the apical pole of the thyrocyte. Secondly, after endocytosis thyroglobulin is lysed and T3 and T4 are secreted at the basal pole into the bloodstream. However, usually exocrine glands are constituted of 2 well separate components: an acinus/alveolar component and an exocrine duct component. Under particular conditions such as chronic injury the acinus/alveolar component is rapidly destroyed, whereas the ductal component seems to be far more resistant and can proliferate giving rise to a tubular network described as "ductulus reaction" or "ductal metaplasia." Normal exocrine ducts and metaplastic ducts exhibit common genetic and phenotypic features directly related to their tubular morphology. In this study, we describe in lymphocytic autoimmune thyroiditis the appearance of ductal-like structures which displayed the features of ductal metaplasia.
[Show abstract][Hide abstract] ABSTRACT: To analyze the immunocytochemical distribution of CK19 and p63 on archival cytologic smears of 27 papillary thyroid carcinomas (PTCs), 22 benign thyroid lesions and 5 malignant non-PTC lesions.
Archival cytologic smears of 27 papillary carcinomas, 22 benign thyroid lesions and 5 malignant nonpapillary carcinomas were processed for immunocytochemical detection of CK19 and p63, and results were compared.
CK19 showed strong cytoplasmic staining in 22/27 fine needle aspiration biopsies (FNABs) of PTCs, in 5 benign lesions and in 4 malignant lesions of the control group. p63 Positivity was present in FNABs of 20/27 PTC and in 1 FNAB of nodular hyperplasia. Eighteen FNABs of PTC (66.6%) showed both strong CK19 staining and p63-positive cells, whereas none of the control cases showed coexpression of CK19 and p63.
Coexistence of strong CK19 positivity and p63-positive cells can enhance the cytologic diagnosis of PTC.
[Show abstract][Hide abstract] ABSTRACT: We describe a case of solid cell nest hyperplasia associated with papillary thyroid carcinoma in a 48-year-old man with goiter. The entire gland was examined; in 1 section, the cells of 1 solid cell nest were in close contact with a follicular variant of papillary microcarcinoma. A second follicular variant of papillary microcarcinoma, 1 follicular adenoma, hyperplastic nodules, and some lymphoid aggregates were also found. Scattered p63-positive cells were found in the second papillary microcarcinoma. After microdissection, the same BRAF(V600E) mutation was found both in a pool of 5 solid cell nests and in the adjacent papillary microcarcinoma. BRAF(V600E) mutation and the previously unreported BRAF(G593D) mutation along with p.G606G silent change were found in the second papillary microcarcinoma, but no mutations were detected in the follicular adenoma or in the 2 other pools of solid cell nests screened for BRAF gene mutations. These findings support a histogenetic link between the main cells of solid cell nests and papillary thyroid carcinoma, and suggest solid cell nest hyperplasia as a precursor lesion of papillary thyroid carcinoma.
Human pathology 04/2009; 40(7):1029-35. DOI:10.1016/j.humpath.2008.11.015 · 2.77 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We report the first case of oncocytic solid cell nests (SCNs), found in the right lobe of the thyroid of a 70-year-old man. Conventional SCNs and 1 papillary microcarcinoma (mPTC) were also found in the left lobe. In the oncocytic SCNs, 80% of the main cells showed oncocytic cytoplasm immunoreactive for porin and proteins of the SDHB and SDHA genes. Positivity for cytokeratin 19, p63, galectin-3, and HBME-1 and negativity for thyroglobulin, thyroperoxidase, vimentin, Oct-4, and α-fetoprotein were found in oncocytic and conventional SCNs. An inverse correlation was found between oncocytic metaplasia and p63. Association with C cells was confirmed at protein and messenger RNA levels in both types of SCNs. No germinal mutation of GRIM-19 was detected. No somatic BRAF mutation was found in any of the SCNs nor in the mPTC. We conclude that SCNs may acquire mitochondrial alterations similar to those seen in follicular and C cells, as well as in their respective tumors.
American Journal of Clinical Pathology 04/2012; 137(4):612-8. DOI:10.1309/AJCPB0RXYPACLL5K · 2.51 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.