Meiotic competence of prepubertal goat oocytes.
ABSTRACT The object of this work was to evaluate in vitro maturation of follicular oocytes from the ovaries of prepubertal goats obtained from the slaughterhouse. To obtain the oocytes, follicles were dissected and classified according to their diameters. In the first experiment, oocytes were matured in vitro with granulosa cells. No significant differences were detected in the percentages of maturation between adult and prepubertal goat oocytes recovered from follicles of 2.5 to 6.0 mm in diameter (81.82 vs 72.47%, respectively). The percentage of maturation increased to 88.0% in prepubertal goat oocytes from 3.0 to 6.0-mm follicles. In the second experiment, the percentage of maturation of prepubertal goat oocytes was greater after 27 than after 24 h. In the third experiment, the maturational capacity of prepubertal goat oocytes according to follicular diameter was evaluated. The percentages of maturation after 27 h of culture with no granulosa cells were 24.14, 56.60 and 74.78%, respectively, for follicles 1.0 to 1.9 mm, 2.0 to 2.9 mm, and 3.0 to 6.0 mm in diameter. As the follicular diameter increased, growth of the oocyte as well as a greater number of oocytes with more cumulus cell layers were observed. A correlation between the diameter of the oocyte and its competence to complete in vitro maturation was also observed. Oocytes with more cumulus cell layers showed only a slight superiority in their capacity for maturation in large-size follicles (3.0 to 6.0 mm), but the difference was not significant. In conclusion, oocytes from prepubertal goats complete their growth and reach meiotic competence in follicles larger than 3.0 mm. With these oocytes it is possible to obtain in vitro maturation results similar to those from adult goats.
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ABSTRACT: The present study was aimed at assessing efficacy of 3 harvesting methods on the quantity and quality of oocytes recovered for assisted reproduction procedures in goat. The average total number of oocytes recovered per ovary was significantly higher by slicing (6.54±0.39) and puncture (6.59±0.39) than by the aspiration method (4.09±0.19). However, the percentage of good quality oocytes was higher in the puncture method (71.56%), compared to the aspiration (71.27%) or slicing (61.61%) methods. The oocyte recovery was significantly lower in CL containing ovaries than that of ovaries without CL in aspiration (2.92 vs 4.57), puncture (5.89 vs 6.78) and slicing (5.40 vs 7.02) methods. However, the presence of CL did not affect the oocytes ability to reach the MII stage (75.31% vs 76.67%). The side (right or left) of the ovary not showed any significant effect on mean of total oocyte recovery and other grades of oocytes. However the large sized ovaries were yielded significantly higher number of oocytes than smaller ovaries. The results showed that the rates of COCs that reached the metaphase-II (M-II) stage were 79.88, 78.09 and 72.63% in aspiration, puncture and slicing techniques, respectively. The maturation rate was significantly lower in slicing method. The oocytes obtained were matured and parthenogenetically activated in vitro using ionomycin and 6-DMAP. There was no significant difference in the subsequent percentage of cleavage and development to blastocyst stage in vitro between the three methods of oocyte harvesting. It was concluded that though oocyte recovery, and in vitro developmental rates did not vary significantly between puncture and slicing methods, yet puncture method was found to be superior due to low debris content and recovery of more number of culture grade oocytes. Oocyte recovery by aspiration from small sized ovaries was difficult due to less number of visible follicles. Puncture method can be used as an alternative to slicing and aspiration for oocyte recovery in goat.The Indian journal of animal sciences 10/2013; 83(10):1042. · 0.13 Impact Factor
- Resuscitation 01/2010; 81(2). · 4.10 Impact Factor
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ABSTRACT: Oocytes are the main raw materials for in vitro embryo production (IVP) experiments. Therefore, the success of any IVP program in goat production, either in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) largely depends on the continuous supply of quality oocytes in optimum quantity. A number of methods are currently used for oocyte recovery (OR) from live or slaughtered goats. Although, abattoir is the most easy and cheapest source of oocytes, however, in certain countries especially in Malaysia, abattoir source is extremely limited due to low slaughtering activities as a consequence of shortage of breeding stock. In Malaysia normally older goats or goats those are culled for breeding are slaughtered for meat. Therefore, the quality of oocytes recovered from ovaries of these goats is generally lower. On the contrary, OR from live goats using laparoscopic ovum pick-up (LOPU) technique provides many advantages, for example, OR can be repeated 3-5 times in the same goat at interval as short as a week or less, OR can be done in prepubertal and aged goats, if prepubertal goat is used for OR then generation intervals will be reduced, LOPU coupled with IVP can become an effrcient method of early propagation of valuable goats and LOPU can overcome limitations frequently associated with multiple ovulation-embryo transfer (MOET). Therefore, LOPU can be an alternative and efficient OR method in goat not only for Malaysia but also for other countries. The current study will discuss oocyte recovery methods with special reference to LOPU and grading of recovered oocytes in goats. © 2008 Asian Network for Scientific Information.Biotechnology. 01/2008; 7(4):612-622.