Validation of a rapid enzyme immunoassay for the quantitation of retinol-binding protein to assess vitamin A status within populations.

Formerly of Program for Appropriate Technology in Health (PATH), Seattle, WA 98396, USA.
European Journal of Clinical Nutrition (Impact Factor: 2.95). 11/2006; 60(11):1299-303. DOI: 10.1038/sj.ejcn.1602456
Source: PubMed

ABSTRACT To compare the prevalence of vitamin A deficiency (VAD) among Cambodian preschool children as determined by the retinol-binding protein-enzyme immunoassay (RBP-EIA) and direct measurement of serum retinol by high-performance liquid chromatography (HPLC).
Sera from 359 children were randomly selected from archived specimens collected in a national VAD prevalence survey in Cambodia.
Sera were first analyzed for retinol content by HPLC and then subjected to analysis using RBP-EIA to determine serum RBP concentrations. National Institute of Standards and Technology and control sera were used to ensure quality and accuracy for each set of analyses. To classify VAD, the same cutoff point of <0.70 micromol/l was employed for each indicator.
Overall, the prevalence of VAD based on serum retinol was 22.3% (95% confidence interval (CI): 18.0, 26.6), whereas the RBP-EIA indicated a VAD prevalence of 20.9% (95% CI: 16.7, 25.1). A simple linear regression model indicated an R2 of 0.79, and a receiver operating curve analysis revealed an area under the curve of 0.92.
We found no significant difference between the results of RBP-EIA compared to retinol analyzed by HPLC in estimating the prevalence of VAD. Use of the test could enable public health authorities to assess the extent of VAD and track progress in control programs in resource-poor settings.

1 Follower
  • [Show abstract] [Hide abstract]
    ABSTRACT: The vitamin A deficiency (VAD) is considered as a major public health problem in the world. This phenomenon is still not well known in Algeria. The prevalence of this deficiency is calculated on a group of children in good health, not supplemented with vitamin A, from rural, semi-rural and urban environments in the Blida region (Algeria), aged 1 to 23 months and recruited in the pediatric services of Blida area, during the period of November 2007–April 2008. The sample is composed of 150 children (87 boys and 63 girls) who present a concentration of C-reactive protein (CRP) < 10 mg/L. The mean concentrations of serum retinol are 1.049 ± 0.422 μmol/L. The dosages of the serum retinol-binding protein (RBP) and prealbumin revealed the mean values of 0.024 ± 0.009 g/L and of 0.152 ± 0.039 g/L, respectively for RBP and prealbumin. The prevalence of serum retinol deficiency is 19%; this indicates the presence of a moderate VAD. The prevalence of carrier protein deficiency is 76% for the RBP and 10% for the prealbumin. To remedy the problem of vitamin A deficiency, it is advisable to implement a policy of nutritional education. The supplementation can be interesting only for the children whose nutritional status is defective or living in areas where the food products rich in vitamin A are rare.
    Nutrition Clinique et Métabolisme 02/2014; DOI:10.1016/j.nupar.2013.11.002 · 0.62 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: OBJECTIVE To evaluate the effectiveness of Sprinkles alongside infant and young child feeding (IYCF) education compared with IYCF education alone on anemia, deficiencies in iron, vitamin A, and zinc, and growth in Cambodian infants. DESIGN Cluster-randomized effectiveness study. SETTING Cambodian rural health district. PARTICIPANTS Among 3112 infants aged 6 months, a random subsample (n = 1350) was surveyed at baseline and 6-month intervals to age 24 months. INTERVENTION Daily micronutrient Sprinkles alongside IYCF education vs IYCF education alone for 6 months from ages 6 to 11 months. MAIN OUTCOME MEASURES Prevalence of anemia; iron, vitamin A, and zinc deficiencies; and growth via biomarkers and anthropometry. RESULTS Anemia prevalence (hemoglobin level <11.0 g/dL [to convert to grams per liter, multiply by 10.0]) was reduced in the intervention arm compared with the control arm by 20.6% at 12 months (95% CI, 9.4-30.2; P = .001), and the prevalence of moderate anemia (hemoglobin level <10.0 g/dL) was reduced by 27.1% (95% CI, 21.0-31.8; P < .001). At 12 and 18 months, iron deficiency prevalence was reduced by 23.5% (95% CI, 15.6-29.1; P < .001) and 11.6% (95% CI, 2.6-17.9; P = .02), respectively. The mean serum zinc concentration was increased at 12 months (2.88 μg/dL [to convert to micromoles per liter, multiply by 0.153]; 95% CI, 0.26-5.42; P = .03). There was no statistically significant difference in the prevalence of zinc and vitamin A deficiencies or in growth at any time. CONCLUSIONS Sprinkles reduced anemia and iron deficiency and increased the mean serum zinc concentration in Cambodian infants. Anemia and zinc effects did not persist beyond the intervention period. TRIAL REGISTRATION Identifier: ACTRN12608000069358.
    JAMA Pediatrics 09/2012; 166(9):842-50. DOI:10.1001/archpediatrics.2012.1003 · 4.25 Impact Factor
  • Source

Full-text (2 Sources)

Available from
Oct 16, 2014