Glycine reduces tissue lipid peroxidation in hypoxia-reoxygenation-induced necrotizing enterocolitis in rats.

Experimental Surgery, UNIFESP, Brazil.
Acta Cirurgica Brasileira (Impact Factor: 0.57). 01/2006; 21(3):161-7. DOI: 10.1590/S0102-86502006000300008
Source: PubMed

ABSTRACT To assess the protective effect of glycine in an experimental model of Neonatal Necrotizing Enterocolitis (NEC).
Fifty (50) neonatal Wistar rats, from a litter of six female rats and weighing 4 to 6 grams, were used. Five animals were cannibalized and the 45 remaining were distributed into three groups: the G1 normal control group (n=12); the G2 Group (n=16), of animals that underwent hypoxia-reoxygenation (HR); the G3 Group of animals (n=17) that underwent HR following a 5% intraperitoneal glycine infusion. The animals underwent hypoxia in a CO2 chamber receiving an air flow of 100% CO2 for 5 minutes and reoxygenation receiving an O2 flow at 100% for 5 minutes. One centimeter long small bowel and colon segments were prepared for histological analysis. The rest of the bowel was removed in a block and frozen at minus 80 degrees C for homogenization and determination of tissue malondialdehyde (MDA). Tissue lesions were classified as Grade 0 to Grade 5, according to the level of damaged mucosa.
The animals in Group G1 had levels of small bowel and colon lesion significantly smaller as compared to the animals in Groups G2 and G3. The G2 group had mean MDA values significantly higher than the animals in the G1 (p = .015) and G3 (p=0.021) groups. MDA values did not differ significantly (p = 0.992) for the animals in groups G1 and G3.
Glycine reduces tissue MDA levels (a measurement of lipid peroxidation) following HR in neonatal rats.

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    ABSTRACT: Objective: To assess the protective effect of glycine in liver of neonatal hypoxia/reoxygenation-induced necrotizing enterocolitis in rats. Methods: Forty-four (44) neonatal Wistar rats were distributed into three groups: G1 - normal control group (n=12); G2 Group (n=16) with animals that underwent hypoxia- reoxygenation; G3 Group (n=17) with animals submitted to hypoxia-reoxygenation following a 5% intraperitoneal glycine infusion. The groups were subdivided into A: euthanasia 12 h after hypoxia-reoxygenation and B: euthanasia 72 h after hypoxia- reoxygenation. The liver was removed for determination of tissue malondialdehyde. Results: Malondialdehyde values did not differ significantly in subgroups G1 and G3. The animals in G2A had mean malondialdehyde values significantly lower than those in G2B. Malondialdehyde values did not differ significantly for animals in subgroup A. In subgroup B, the malondialdehyde values did not differ significantly among the animals in G1 and G3. G2 animals had mean malondialdehyde values significantly higher than G3 animals. Conclusion: Glycine reduces liver lipid peroxidation in hypoxia/reoxygenation-induced necrotizing enterocolitis.


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Sep 17, 2014