Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis

The Department Cell Biology and Medical Scientist Training Program, The University of Alabama at Birmingham, VH 648A, 1670 University Blvd., Birmingham, Alabama 35294-0019, USA.
The FASEB Journal (Impact Factor: 5.04). 07/2006; 20(8):1045-54. DOI: 10.1096/fj.05-5460rev
Source: PubMed

ABSTRACT Thy-1 (CD90) is a 25-37 kDa glycosylphosphatidylinositol (GPI) -anchored glycoprotein expressed on many cell types, including T cells, thymocytes, neurons, endothelial cells, and fibroblasts. Activation of Thy-1 can promote T cell activation, and this role of Thy-1 is reviewed elsewhere. Thy-1 also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death. In reviewing the nonimmunologic functions of Thy-1, we discuss the phenotype of the Thy-1 null mouse, signaling pathways modulated by Thy-1, the role of the GPI anchor in Thy-1 localization to lipid rafts and signaling, and regulation of Thy-1 expression. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.

Download full-text


Available from: James S Hagood, Sep 24, 2014
27 Reads
  • Source
    • ", cancer stem cells (CSCs) [54] CD13 (aminopeptidase N) CSCs [55], myeloid cells [56] CD29 (integrin beta-1) NSCs [57], CSCs [58] CD44 T cells [59], CSCs [60] [61] CD73 (NT5E) Endothelial cells [62], lymphocytes [63] CD90 (Thy-1) T cells [64] CD105 (endoglin) HSCs [65], endothelial cells [66], macrophages [67] CD106 (VCAM-0031) Endothelial cells [68] CD146 (MCAM) T cells [69], pericytes [70], endothelial cells [71] CD166 (ALCAM) Epithelial cells [72] [73] CD271 (LNGFR) NSCs [74], CSCs [75] PDGFRí µí»¼ (CD140a) Fibroblasts [76], smooth muscle cells [77] Leptin R Adipocytes [78] have shown that cells that expressed the Nestin-GFP (Nes- GFP) transgene behave functionally as MSCs and are closely associated with HSC quiescence and maintenance in bone marrow [30] [31] [32]. A recent study, however, reported that Nes + cells could mark both endothelial and nonendothelial cells during endochondral ossification [33]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into multiple cell lineages and contributing to tissue repair and regeneration. Characterization of the physiological function of MSCs has been largely hampered by lack of unique markers. Nestin, originally found in neuroepithelial stem cells, is an intermediate filament protein expressed in the early stages of development. Increasing studies have shown a particular association between Nestin and MSCs. Nestin could characterize a subset of bone marrow perivascular MSCs which contributed to bone development and closely contacted with hematopoietic stem cells (HSCs). Nestin expressing (Nes(+)) MSCs also play a role in the progression of various diseases. However, Nes(+) cells were reported to participate in angiogenesis as MSCs or endothelial progenitor cells (EPCs) in several tissues and be a heterogeneous population comprising mesenchymal cells and endothelial cells in the developing bone marrow. In this review article, we will summarize the progress of the research on Nestin, particularly the function of Nes(+) cells in bone marrow, and discuss the feasibility of using Nestin as a specific marker for MSCs.
    Stem cell International 08/2015; 2015:762098. DOI:10.1155/2015/762098 · 2.81 Impact Factor
    • "These data suggest different cell type-or contextdependent functions for Thy-1. In contrast to multiple reports on the functional role of Thy-1 to proliferation, only few studies examined its contribution to apoptotic signaling (Rege and Hagood, 2006). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Fibroblasts provide matrix and regulatory mediators to the microenvironment and thereby contribute to maintenance of tissue homeostasis, wound healing and tumor progression. In the present study we addressed the functional relevance of Thy-1 for fibroblast functions in vitro and in vivo. Using fibroblasts from Thy-1(-/-) and wildtype mice, recombinant expression of Thy-1 and analysis of the interaction of fibroblasts with immobilized Thy-1 we showed that Thy-1 plays a crucial role in the control of cell growth by suppressing proliferation and promoting apoptosis and differentiation of dermal fibroblasts. Function-blocking studies revealed that Thy-1 mediates the control of apoptosis and proliferation via modulation of β3 integrin function. Interestingly, Thy-1-mediated growth control appears to be a more general mechanism because it also regulates growth of tumor cells of different origin dependent on their β3 integrin expression. In summary, our findings point to an important role of Thy-1 in controlling the balance between proliferation and differentiation in dermal fibroblasts.Journal of Investigative Dermatology accepted article preview online, 04 March 2015. doi:10.1038/jid.2015.86.
    Journal of Investigative Dermatology 03/2015; 135(7). DOI:10.1038/jid.2015.86 · 7.22 Impact Factor
  • Source
    • "We questioned as to whether pPiPSCs retained these favorable characteristics after reprogramming. ADSCs strongly expressed CD29, CD44, and CD90, which are vital for the regulation of proliferation, differentiation, adhesion, migration, and tumor suppression; owing to the expression of these factors, ADSCs have become favorable candidates for clinical application (Chang et al. 1997, Rege & Hagood 2006, Sackstein et al. 2008). pPiPSCs and pPiPSC-DCs expressed high levels of these molecules, demonstrating that these cell lines also possess these abilities. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Partially reprogrammed induced pluripotent stem cells (PiPSCs) have great potential for investigating reprogramming mechanisms, and represent an alternative potential material for making genetically modified animals and regenerative medicine. To date, PiPSCs have scarcely been detailed reported other than mice and humans. Here, we obtained PiPSCs from porcine adipose-derived stem cells (pADSCs) by ectopic expression of human transcription factors (OCT4, SOX2, c-MYC and KLF4) in feeder-free condition. The morphology and proliferation activity of porcine PiPSCs (pPiPSCs) were similar to those of porcine fully reprogrammed iPSCs (pFiPSCs); furthermore, pPiPSCs expressed higher levels of the typical surface molecules (CD29) found in pADSCs. However, pPiPSCs were negative for key proteins (NANOG) connected with stemness, and possessed lower differentiation ability in vivo and in vitro. When differentiation-inhibiting factors were withdrawn, pPiPSCs-derived cells (pPiPSC-DCs) showed similar features with pADSCs in many aspects, including proliferation, differentiation, and immunosuppression. When both types of cells were used to produce cloned embryos, we found that the blastocyst rate of 19DC (one of the pPiPSC-DC cell lines)-derived cloned embryos was obviously higher than that of others. The total cell number of 19DCs-derived blastocysts was significantly higher than the 30DC (one pFiPSC-DC cell line)-derived blastocysts. In all, through limited differentiation ability, the proliferation activity of pPiPSCs is similar to that of pFiPSCs, and pPiPSCs can retain several of the features of pADSCs, which are beneficial to cell therapy. Furthermore, the differentiation of pPiPSCs is more favorable for producing high-quality reconstructed embryos.
    Reproduction (Cambridge, England) 02/2015; 149(5). DOI:10.1530/REP-14-0410 · 3.17 Impact Factor
Show more