Matrine-induced apoptosis in leukemia U937 cells: Involvement of caspases activation and MAPK-independent pathways

Center for Molecular Biology, School of Medicine, Shantou University, Shantou, P. R. China.
Planta Medica (Impact Factor: 2.34). 06/2006; 72(6):501-6. DOI: 10.1055/s-2006-931534
Source: PubMed

ABSTRACT It is reported that matrine, one of the major effective compounds isolated from the root of Sophora flavescens Ait., has anti-leukemia activity. In this study, we find that the treatment of leukemia U937 cells with matrine results in induction of apoptosis. Analysis of the mechanism underling this apoptotic event showed activation of caspases-9, -3, and -7, and release of cytochrome C from mitochondria to cytosol, and cleavage of poly(ADP-ribose) polymerase. Matrine did not alter the level of bcl-2 and bcl-xL as well as bax. In addition, no correlation was found between matrine administration and activation of the three major MAPK subfamilies (Erk1/2, p38, JNK/SAPK). The results indicate that matrine induces apoptosis in U937 cells via a cytochrome C-triggered caspase activation pathway.

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    • "Matrine, one of the main active components from the dry roots of Sophora flavescence (SF), has long been used for treatment of cancer, viral hepatitis, arrhythmia and skin diseases in China (Zhang and Huang, 2004). Some studies indicated that Matrine could induce leukemia U937, JM, K562 cells apoptosis and cell cycle arrest in vitro (Liu et al., 2006; Feng et al., 2003; Zhang et al., 2001). However, the mechanism of underlying the apoptotic process by Matrine remains to be elucidated. "
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