Structure of the Vesicular Stomatitis Virus Nucleoprotein-RNA Complex

Department of Microbiology, School of Medicine, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA.
Science (Impact Factor: 33.61). 08/2006; 313(5785):357-60. DOI: 10.1126/science.1126953
Source: PubMed


Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved
in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a
complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface
between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA
to be accessed, some conformational change in the N protein should be necessary.

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    • "The N ORFs each encode 434 aa mildly acidic polypeptides (ABTV, 49.7 kDa; PTAMV, 49.9 kDa) that share 38.6 % overall amino acid sequence identity. A CLUSTAL_X alignment of their N proteins with that of Vesicular stomatitis Indiana virus (VSIV) indicated preservation of several known conserved motifs, as well as each of the eight basic residues located in the RNA-binding cavity that are known to coordinate binding to viral genomic RNA in the ribonucleoprotein (RNP) (Green et al., 2006; Luo et al., 2007) (Fig. S2). "
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    Journal of General Virology 01/2014; 95(Pt_4). DOI:10.1099/vir.0.058685-0 · 3.18 Impact Factor
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    • "These viruses assemble their RNA replication/transcription complexes in close association with membranous cell compartments that provide an effective shield against dsRNA sensors [2]. -ssRNA viruses carry their own RdRp and prevent the exposure of duplex RNA by protecting their genomic RNA with nucleocapsid proteins, and by assembling transcriptionally active ribonucleoprotein (RNP) complexes [3]. Finally, prototypical dsRNA viruses enclose their genomes in a highly conserved structure, the so-called T = 2 capsid or transcriptional core, that contains all the enzymatic machinery required to synthesize and extrude translatable mRNAs into the cytoplasm of the infected cell. "
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    PLoS ONE 01/2014; 9(1):e87790. DOI:10.1371/journal.pone.0087790 · 3.23 Impact Factor
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    • "The ring-shaped RNPs of respiratory syncytial, rabies, and vesicular stomatitis viruses contain 10–11 N protein molecules (Albertini et al., 2006; Green et al., 2006; Tawar et al., 2009), whereas the N protein of Borna disease virus was crystallized as a tetramer (Rudolph et al., 2003). In most viruses, RNA was observed inside the N ring-structures. "
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