The effect of combinations of flurbiprofen, low molecular weight heparin, and doxycycline on the inhibition of corneal neovascularization

University of Illinois at Chicago, Chicago, Illinois, United States
Cornea (Impact Factor: 2.36). 07/2006; 25(5):582-5. DOI: 10.1097/01.ico.0000220767.73660.3a
Source: PubMed

ABSTRACT To determine the efficacy of various combinations of flurbiprofen, low molecular weight heparin (LMWH), and doxycycline on the inhibition of corneal neovascularization in rats.
Chemical cauterization of the cornea in 32 eyes of 32 rats was performed using silver nitrate/potassium nitrate sticks. The animals were divided into 4 groups. Topical instillation of 0.015% flurbiprofen with 5 mg/mL LMWH (group 1); flurbiprofen with 10 mg/mL doxycycline (group 2); doxycycline with LMWH (group 3), and saline (group 4; control) was performed twice a day for 7 days. Slit-lamp photography was used to determine the percent areas of cornea covered by neovascularization and also by scar in each group. The means were compared to find any significant (P < 0.05) difference between groups. The animals were euthanized and the eyes enucleated for histological evaluation.
The mean of percent area of corneal neovascularization 5 in group 1 was 48.5 +/- 13.1; group 2, 6.6 +/- 5.5; group 3, 22.0 +/- 27.6 and group 4, 64.6 +/- 9. The means of percent area of neovascularization in groups 2 and 3 were significantly lower compared with control and group 1. There was no significant difference in the percent corneal neovascularization between groups 2 and 3 or between group 1 and the control group. Histological findings were consistent with slit-lamp evaluations.
Topical instillation of combinations of doxycycline with either flurbiprofen or LMWH can effectively inhibit corneal neovascularization made by chemical cauterization of the cornea in rats.

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    • "Therapies inhibiting MMP-9 are thus believed to have potential for inhibiting neovascularization. Previously, it has been shown that tetracycline and its derivatives inhibit corneal neovascularization via suppressing MMP-9 activity, a role that is distinct from its antimicrobial activity [8] [9] [10] [11]. Tigecycline is a newer and more powerful agent of the tetracycline family, which is available as an injectable antibiotic [12]. "
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    • "Various compounds have been identified as inhibitors in experimental and clinical corneal NV. These include steroids , non-steroidal anti-inflammatory drugs, heparin, doxycycline and cyclosporine A, methotrexate and thalidomide (Benelli et al. 1997; Kruse et al. 1998; Joussen et al. 1999; Peyman et al. 2006; Riazi-Esfahani et al. 2006). Topical steroids are still the mainstay of the prevention and treatment of corneal NV, but they may be associated with serious complications such as cataract, glaucoma and infections (Fraunfelder & Rich 2003). "
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