Article

Antibody microarrays using resonance light-scattering particles for detection.

Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA.
Methods in molecular biology (Clifton, N.J.) 02/2006; 328:31-50. DOI:10.1385/1-59745-026-X:31 pp.31-50
Source: PubMed

ABSTRACT Antibody microarray measurements show great potential for the simultaneous quantification of many proteins in small amounts of body fluids and extracts. Over the last few years, a micro-array platform centered around the concept of microarrays in microtiter wells was developed, and for the best assays we have achieved lower limits of detection in the femtomolar range using resonance light-scattering particles for the staining of biotinylated detection antibodies. Although conceptually simple, these multiplexed sandwich assays are technically challenging. Here we describe in detail our protocols and procedures for the manufacturing of antibody microarrays with up to 48 different antibodies and for performing plasma measurements.

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Keywords

48 different antibodies
 
Antibody microarray measurements
 
antibody microarrays
 
biotinylated detection antibodies
 
detection
 
lower limits
 
micro-array platform
 
microarrays
 
microtiter wells
 
proteins
 
protocols
 
resonance light-scattering particles
 
simultaneous quantification
 
small amounts
 
staining
 

Bernhard H Geierstanger