PCR amplification of species specific sequences of 16S rDNA and 16S-23S rDNA intergenic spacer region for identification of Streptococcus phocae.

Institut für Tierärztliche Nahrungsmittelkunde, Professur für Milchwissenschaften, Justus-Liebig-Universität Giessen, Ludwigstr. 21, 35390 Giessen, Germany.
Microbiological Research (Impact Factor: 1.94). 02/2008; 163(2):132-5. DOI: 10.1016/j.micres.2006.01.017
Source: PubMed

ABSTRACT Streptococcus phocae, a bacterial pathogen of seals, could reliably be identified by PCR amplification using oligonucleotide primers designed according to species specific segments of the previously sequenced 16S rRNA gene and the 16S-23S rDNA intergenic spacer region of this species. The PCR mediated assay allowed an identification of S. phocae isolated from harbor and gray seals and from Atlantic salmons. No cross-reaction could be observed with 13 different other streptococcal species and subspecies and with Lactococcus garvieae strains investigated for control purposes.

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