Hymenolepis nana infection: symptoms and response to nitazoxanide in field conditions.
ABSTRACT A matched case-control study on clinical symptoms of Hymenolepis nana and an open trial of nitazoxanide treatment were conducted in a village on the northern coast of Peru. Interview data were obtained from 25 individuals with hymenolepiasis only and 46 matched controls. Individuals with other intestinal helminths or giardiasis were excluded. Twenty nitazoxanide-treated cases were followed with stool examination a month later. There were no significant differences between groups in the prevalence of symptoms except headache, which was less frequent in the cases. Treatment efficacy was 75%. Hymenolepiasis in this community did not have significant impact on specific symptoms.
Article: TsAg5, a Taenia solium cysticercus protein with a marginal trypsin-like activity in the diagnosis of human neurocysticercosis.[show abstract] [hide abstract]
ABSTRACT: Neurocysticercosis is an endemic parasitic disease caused by Taenia solium larva. Although the mechanism of infection is not completely understood, it is likely driven by proteolytic activity that degrades the intestinal wall to facilitate oncosphere penetration and further infection. We analyzed the publicly available T. solium EST/DNA library and identified two contigs comprising a full-length cDNA fragment very similar to Echinococcus granulosus Ag5 protein. The T. solium cDNA sequence included a proteolytic trypsin-like-domain in the C-terminal region, and a thrombospondin type-1 adherence-domain in the N-terminal region. Both the trypsin-like and adherence domains were expressed independently as recombinant proteins in bacterial systems. TsAg5 showed marginal trypsin-like activity and high sequence similarity to Ag5. The purified antigens were tested in a Western immunoblot assay to diagnose human neurocysticercosis. The sensitivity of the trypsin-like-domain was 96.36% in patients infected with extraparenchymal cysts, 75.44% in patients infected with multiple cysts, and 39.62% in patients with a single cyst. Specificity was 76.70%. The thrombospondin type-1 adherence-domain was not specific for neurocysticercosis.Molecular and Biochemical Parasitology 08/2011; 180(2):115-9. · 2.55 Impact Factor