Study on biological variation of haemostatic parameters in clinically healthy dogs

Novo Nordisk, København, Capital Region, Denmark
The Veterinary Journal (Impact Factor: 1.76). 08/2007; 174(1):62-8. DOI: 10.1016/j.tvjl.2006.05.003
Source: PubMed


Thromboelastography (TEG) may be a valuable supplement to the coagulation assays activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), fibrinogen, antithrombin (AT) and D-Dimer currently used in most clinical pathology laboratories. Allowable imprecision and bias reference limits for analytical tests can be calculated based on measurements of biological variation. No studies to date have examined the effect of biological variation on these haemostasis parameters in the same group of dogs. Plasma samples were collected after a set protocol once weekly for five consecutive weeks from eight healthy dogs (four males and four females) and stored at -80 degrees C until analysis. Randomized duplicate coagulation tests and TEG analyses were performed on all plasma samples within one run. The data were analyzed for outliers and subsequently subjected to nested analysis of variance to obtain the coefficient of analytical, intra-individual and inter-individual variation. From these objective analytical performance standards for imprecision, critical difference, total error and the index of individuality were calculated to assess the utility of conventional population-based reference ranges. All the clotting times (aPTT, PT and TT), fibrinogen, AT and D-Dimer showed a degree of individuality, which may make the use of population-based reference ranges alone an insensitive interpretation criterion, whereas a population-based reference interval seems to be sensitive for interpreting all TEG parameters. Analytical performance standards for imprecision were only met for one of the coagulation assays, whereas all TEG parameters except the alpha angle, alpha achieved this analytical goal.

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    • "TEG analyses were performed on citrated plasma samples as previously described [18], using a computerized thromboelastograph (TEG 5000 Hemostasis Analyzer System, Haemonetics) with continuous data acquisition. In brief, samples were thawed in a water bath at 37°C, and activated using a solution of recombinant human tissue factor (TF) (Innovin, Dade Behring) at a final TF dilution of 1:50,000 [18]. "
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    ABSTRACT: Sled dogs performing endurance races have been reported to have a high incidence of gastric erosions or ulcerations and an increased risk of gastro intestinal bleeding leading to death in some cases. In addition, these dogs also become hypothyroid during training and exercise. Canine hypothyroidism has been shown to correlate with decreased von Willebrand factor antigen and potentially increased bleeding tendency. Whether increased gastro intestinal bleeding risk is exacerbated due to changes in the hemostatic balance in unknown. The aim of this study was to investigate the hemostatic balance in sled dogs before and after exercise and in addition evaluate any correlation to thyroid status. Twenty sled dogs have been assessed in untrained and trained condition and immediately after exercise. The first sample was collected in the autumn following a resting period, and subsequently the dogs were exposed to increased intensity of training. After four months the peak of physical condition was reached and a 68 km long sled pulling exercise was performed. Samples were collected before and immediately after the exercise. Evaluated parameters were: plasma thromboelastographic (TEG) R, SP, alpha and MA, activated partial thromboplastin time (aPTT), prothrombin time (PT), fibrinogen, von Willebrand factor (vWf), D-dimer, platelet number, thyroid hormones, hematocrit and C-reactive protein (CRP). Exercise induced an overall hypercoaguable state characterized by significant decreases of TEG R and SP and an increase of alpha, increased concentrations of plasma vWf and decreased aPTT. In addition, a proinflammatory status was seen by a significant increase of serum CRP concentrations. Thyroid status was confirmed to be hypothyroid as training and exercise induced significant decrease of thyroxin (T4), free thyroxin (fT4) and thyroxin stimulating hormone (TSH) concentrations. Fibrinogen decreased significantly and PT increased. The training-induced changes showed correlation between T4, fT4 and aPTT and correlation between TSH and fibrinogen. Exercise-induced changes showed correlation between T4 and PT. Exercise was associated with a hypercoaguable state and an increase of vWf concentration in this group of sled dogs. Decreased thyroid hormone concentrations after training and exercise were confirmed, but were associated with increased and not decreased vWf in this group of sled dogs.
    Acta Veterinaria Scandinavica 02/2014; 56(1):11. DOI:10.1186/1751-0147-56-11 · 1.38 Impact Factor
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    • "The plasma coagulation factor activities, PT, aPTT, AT-and PC-activity and fibrinogen concentration were assessed using an automated coagulometric analyser (ACL top 500, Instrumentation Laboratory). Plasma D-dimer concentration was measured using an immunometric flow-through principle (Nycocard Reader II, ILEX) (Wiinberg et al., 2007). The stored citrated plasma samples were transported on dry ice to the Veterinary Clinical Pathology Laboratory, University of Copenhagen, and transit time for the shipment was <24 h. "
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    The Veterinary Journal 10/2012; 196(2). DOI:10.1016/j.tvjl.2012.09.009 · 1.76 Impact Factor
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