Identification of five phytosterols from Aloe vera gel as anti-diabetic compounds.

Biochemical Research Laboratory, Morinaga Milk Industry Co., Ltd, Kanagawa, Japan.
Biological & Pharmaceutical Bulletin (Impact Factor: 1.78). 08/2006; 29(7):1418-22.
Source: PubMed

ABSTRACT The genus Aloe in the family Liliaceae is a group of plants including Aloe vera (Aloe barbadensis MILLER) and Aloe arborescens (Aloe arborescens MILLER var. natalensis BERGER) that are empirically known to have various medical efficacies. In the present study, we evaluated the anti-hyperglycemic effect of Aloe vera gel and isolated a number of compounds from the gel. On the basis of spectroscopic data, these compounds were identified as lophenol, 24-methyl-lophenol, 24-ethyl-lophenol, cycloartanol, and 24-methylene-cycloartanol. These five phytosterols were evaluated for their anti-hyperglycemic effects in type 2 diabetic BKS.Cg-m(+/+)Lepr(db/J) (db/db) mice. In comparison with the hemoglobin A1c (HbA1c) levels of vehicle-treated mice, statistically significant decreases of 15 to 18% in HbA1c levels were observed in mice treated with 1 mug of the five phytosterols. Considering the ability to reduce blood glucose in vivo, there were no differences between the five phytosterols. Administration of beta-sitosterol did not reduce the blood glucose levels in db/db mice. After administration of the five phytosterols for 28 d, fasting blood glucose levels decreased to approximately 64%, 28%, 47%, 51%, and 55% of control levels, respectively. Severe diabetic mice treated with phytosterols derived from Aloe vera gel did not suffer weight reduction due to glucose loss in the urine. These findings suggest that Aloe vera gel and phytosterols derived from Aloe vera gel have a long-term blood glucose level control effect and would be useful for the treatment of type 2 diabetes mellitus.

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    ABSTRACT: Bangladesh has a rich tradition of folk medicinal practices. Folk medicinal practitioners, known otherwise as Kavirajes, administer medicinal plants for treatment of various ailments. The Kavirajes serve as the primary health-care providers to the mainly rural population of Bangladesh as well as to a substantial number of the urban population. Since the selection of medicinal plants to treat ailments varies widely between Kavirajes of various regions, the objective of this present ethnomedicinal survey among the Kavirajes of Noakhali and Feni districts (which are adjoining districts in the southern part of the country) was to study and document the folk medicinal use of plants in these two districts. Interviews were conducted with the help of a semi-structured questionnaire and the guided field-walk method, where the Kavirajes pointed out the medicinal plants and described their uses while taking the interviewer to places from where he usually collected his plants. All plant specimens were identified at the Bangladesh National Herbarium. It was observed that the Kavirajes of Noakhali and Feni districts used 101 plant species for treatment of various ailments. The Acanthaceae, Euphorbiaceae, Leguminosae, Malvaceae, Solanaceae, and Verbenaceae families provided the largest number of plants, the respective numbers being five, five, nine, six, six, and six. Leaves formed the major plant part used (33.3%), followed by whole plants (20.2%) and roots (19.1%). Other plant parts used for treating ailments included stems, barks, flowers, fruits, and seeds. Gastrointestinal disorders (diarrhea, dysentery, indigestion, constipation, bloating) were the most common ailments treated followed by pain, respiratory tract infections (coughs, cold, mucus, asthma), fever, urinary tract infections (e.g. leucorrhea), hepatic disorders (e.g. jaundice), weakness, and rheumatism. It was observed that several plants were used for treatment of cancer, hypertension, diabetes, nerve disorders, helminthiasis, sexually transmitted diseases, tuberculosis, edema, and chicken pox. Two plants were used as contraceptives. Taken together, the plants present considerable potential for scientific studies and discovery of new drugs.
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    ABSTRACT: Pre-diabetes is a disturbing trend in the population, who are at risk of developing type-two diabetes. The aim of this study was to determine the effects use of Aloe vera in different doses on glucose and lipid profile in pre-diabetic subjects. This study was a double blind randomized controlled trial (72 subjects) with pre-diabetes symptoms in 3 groups consumed capsules twice a day: Aloe vera 300 mg (AL300), 500 mg (AL500) and placebo (PL). Fasting blood glucose (FBS), HbA1C and lipid profile were evaluated in baseline, 4 or 8 weeks. On-way ANOVA, Friedman, Wilcoxon, Kruskal-Wallis , Mann-Whitney and Chi-square tests were used for within or between groups statistical analysis. FBS level in group AL300, showed significantly decreased in fourth week after the intervention, compared to PL in the same time (p = 0.001). Also, HbA1C level in this group at the eighth week after the intervention (p = 0.042), had a significant decrease. The levels of Total cholesterol and LDL-C, only in the group AL500 (p < 0.001 and p = 0.01), was significantly reduced, along with HDL-C level improvement just after eight weeks (p = 0.004). Triglyceride level showed a significant decrease (p < 0.045) just after four weeks use of AL500. The Use of Aloe vera extract in pre-diabetic patients, could revert impaired blood glucose within four weeks, but after eight weeks could alleviate their abnormal lipid profile.
    04/2015; 14(1):22. DOI:10.1186/s40200-015-0137-2
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    Biotechnology of Bioactive Compounds: Sources and applications, 1 edited by Vijai Kumar Gupta, Maria G. Tuohy, 01/2015: chapter 23: pages 565-581; John Wiley & Sons, Ltd, Chichester, UK., ISBN: 9781118733103


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