16S rRNA Phylogenetic Investigation of the Candidate Division “Korarchaeota”

Department of Organismic and Evolutionary Biology, Harvard University, Harvard University, Cambridge, MA 02138, USA.
Applied and Environmental Microbiology (Impact Factor: 3.67). 08/2006; 72(7):5077-82. DOI: 10.1128/AEM.00052-06
Source: PubMed


The environmental distribution and phylogeny of “Korarchaeota,” a proposed ancient archaeal division, was investigated by using the 16S rRNA gene framework. Korarchaeota-specific primers were designed based on previously published sequences and used to screen a variety of environments. Korarchaeota 16S rRNA genes were amplified exclusively from high temperature Yellowstone National Park hot springs and a 9°N East Pacific
Rise deep-sea hydrothermal vent. Phylogenetic analyses of these and all available sequences suggest that Korarchaeota exhibit a high level of endemicity.

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    Journal of Proteomics 06/2014; 108. DOI:10.1016/j.jprot.2014.06.011 · 3.89 Impact Factor
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    • "The phylogenetic analysis of the DSAG indicates no apparent habitat-specific clustering of sequences as opposed to findings for other archaeal groups such as the Korarchaeota and the Marine Group I (Auchtung et al., 2006; Durbin and Teske, 2010; Reigstad et al., 2010; Jorgensen et al., 2012). However, sequences in the deepest branching DSAG group (Alpha) are exclusively of hydrothermal origin, suggesting a hydrothermal origin of the entire group, as also previously argued (Reysenbach et al., 2000; Teske et al., 2002). "
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    Frontiers in Microbiology 10/2013; 4:299. DOI:10.3389/fmicb.2013.00299 · 3.99 Impact Factor
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    • "Despite high coverage of the archaeal primers for the Korarchaeota phylum (Figure S1), none of the sequences detected in the OP-YNP sample were classified to this phylum. To evaluate if Korarchaeota were not detected due to the primers designed in this study, we tested the OP-YNP DNA extract with published korarchaeal primers in conjunction with general archaeal primers in the following combination: Kora-228F/Univ-1406R, Kora-228F/Kora-1236R, Arch-4F/Kora-1236R, Arch-112F/Kora-1236R [36], [39]. None of these primer combinations yielded Korarchaeota sequences. "
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    PLoS ONE 08/2012; 7(8):e43093. DOI:10.1371/journal.pone.0043093 · 3.23 Impact Factor
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