Stem Cells: The Real Culprits in Cancer?

University of Michigan, Ann Arbor, USA.
Scientific American (Impact Factor: 1.33). 08/2006; 295(1):52-9. DOI: 10.1038/scientificamerican0706-52
Source: PubMed
  • 10/2010; 1. DOI:10.1016/S2210-7789(10)60177-8
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    ABSTRACT: Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutic target, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells, exhibit a reduced mitochondrial activity and a stronger dependence on aerobic glycolysis. Here, we aimed to comparatively analyze bioenergetics features of the human osteosarcoma 3AB-OS CSC-like line, and the parental osteosarcoma MG63 cells, from which 3AB-OS cells have been previously selected. Our results suggest that 3AB-OS cells depend on glycolytic metabolism more strongly than MG63 cells. Indeed, growth in glucose shortage or in presence of galactose or pyruvate -mitochondrial specific substrates- leads to a significant reduction of their proliferation compared to MG63 cells. Accordingly, 3AB-OS cells show an increased expression of lactate dehydrogenase A (LDHA) and a larger accumulation of lactate in the culture medium. In line with these findings 3AB-OS cells as compared to MG63 cells present a reduced mitochondrial respiration, a stronger sensitivity to glucose depletion or glycolysis inhibition and a lessened sensitivity to oxidative phosphorylation inhibitors. Additionally, in contrast to MG63 cells, 3AB-OS display fragmented mitochondria, which become networked as they grow in glucose-rich medium, while almost entirely loose these structures growing in low glucose. Overall, our findings suggest that 3AB-OS CSCs energy metabolism is more similar to normal stem cells and to cancer cells characterized by a glycolytic anaerobic metabolism. J. Cell. Biochem. © 2013 Wiley Periodicals, Inc.
    Journal of Cellular Biochemistry 02/2014; 115(2). DOI:10.1002/jcb.24671 · 3.37 Impact Factor
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    ABSTRACT: Research with stem cells is the most promising medical discovery for understanding and management of pathologies incurable so far. But besides that, it also announces the birth of regenerative medicine. This article analyses the state of the art with human embryonic and adult stem cells research, from the perspective of its revolutionary biological implications (questioning the classical embryology dogma of modern cardiology as well as the modern neurology dogma, epistemology (insufficient explanation of the mechanistic model of molecular biology) and its bioethical dilemma (moral statute of the embryo, creation of human - non - human and clinical research protocols).
    Acta médica colombiana: AMC: organo de la Asociación Columbiana de Medicina Interna 12/2007; 32(4):231-244.


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