Electrical forces for microscale cell manipulation. Annu Rev Biomed Eng 8:425-454

Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Annual Review of Biomedical Engineering (Impact Factor: 14.21). 02/2006; 8(1):425-54. DOI: 10.1146/annurev.bioeng.8.061505.095739
Source: PubMed


Electrical forces for manipulating cells at the microscale include electrophoresis and dielectrophoresis. Electrophoretic forces arise from the interaction of a cell's charge and an electric field, whereas dielectrophoresis arises from a cell's polarizability. Both forces can be used to create microsystems that separate cell mixtures into its component cell types or act as electrical "handles" to transport cells or place them in specific locations. This review explores the use of these two forces for microscale cell manipulation. We first examine the forces and electrodes used to create them, then address potential impacts on cell health, followed by examples of devices for both separating cells and handling them.

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    • "Dielectrophoretesis (DEP) is an electrical-based technique that has been used for identifying, separating and characterizing single cells [3] [4] [5]. It is an alternative to direct measurement of the dielectric response of a cell in a suspension medium, which requires latter separation of the cell and medium properties. "
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    ABSTRACT: We present a multi-frequency dielectrophoresis (DEP) based microfluidic device for characterizing the complex dielectric properties of single micron-sized particles while in flow. The device employs a multi-electrode transmission line sensor coupled to a microwave-interferometer, capable of subattofarad sensitivity, for detecting the DEP-induced translation of the particle under study. DEP actuation of the particle at different frequencies – which is related to its dielectric response – is sensed as it travels along the sensor. Characterization of the dielectric response of polystyrene micro-spheres using two frequencies is demonstrated.
    International Microwave Symposium (IMS), Pheonix, Arizona, USA; 05/2015
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    • "The current methods commonly used for manipulation of cells employ a variety of mechanical forces originated from centrifugal, hydrodynamic, ultrasonic, optical, electric and magnetic force fields [5] [6] [7] [8] [9] [10] [11]. Among these methods, manipulation using AC-induced dielectrophoretic (DEP) forces may be the most "
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    ABSTRACT: Cell manipulation and separation technologies have potential biological and medical applications, including advanced clinical protocols such as tissue engineering. An aggregation model was developed for a human carcinoma (HeLa) cell suspension exposed to a uniform AC electric field, in order to explore the field-induced structure formation and kinetics of cell aggregates. The momentum equations of cells under the action of the dipole-dipole interaction were solved theoretically and the total time required to form linear string-like cluster was derived. The results were compared with those of a numerical simulation. Experiments using HeLa cells were also performed for comparison. The total time required to form linear string-like clusters was derived from a simple theoretical model of the cell cluster kinetics. The growth rates of the average string length of cell aggregates showed good agreement with those of the numerical simulation. In the experiment, cells were found to form massive clusters on the bottom of a chamber. The results imply that the string-like cluster grows rapidly by longitudinal attraction when the electric field is first applied and that this process slows at later times and is replaced by lateral coagulation of short strings. The findings presented here are expected to enable design of methods for the organization of three-dimensional (3D) cellular structures without the use of micro-fabricated substrates, such as 3D biopolymer scaffolds, to manipulate cells into spatial arrangement.
    Biorheology 03/2015; 51(6). DOI:10.3233/BIR-14034 · 1.18 Impact Factor
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    • "Dielectric properties of a bio-particle depend on the morphology and chemical composition of the internal matrix of the bio-particle. Therefore, each bio-particle has its own dielectric signature [41]. This issue introduces a bio-particle specific selectivity; however, also introduces a challenge. "
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    ABSTRACT: a b s t r a c t Microfluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combi-nation of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manip-ulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.
    Biochemical Engineering Journal 11/2014; 92:63-82. DOI:10.1016/j.bej.2014.07.013 · 2.47 Impact Factor
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