Temperature affects longevity and age-related locomotor and cognitive decay in the short-lived fish Nothobranchius furzeri
ABSTRACT Temperature variations are known to modulate aging and life-history traits in poikilotherms as different as worms, flies and fish. In invertebrates, temperature affects lifespan by modulating the slope of age-dependent acceleration in death rate, which is thought to reflect the rate of age-related damage accumulation. Here, we studied the effects of temperature on aging kinetics, aging-related behavioural deficits, and age-associated histological markers of senescence in the short-lived fish Nothobranchius furzeri. This species shows a maximum captive lifespan of only 3 months, which is tied with acceleration in growth and expression of aging biomarkers. These biological peculiarities make it a very convenient animal model for testing the effects of experimental manipulations on life-history traits in vertebrates. Here, we show that (i) lowering temperature from 25 degrees C to 22 degrees C increases both median and maximum lifespan; (ii) life extension is due to reduction in the slope of the age-dependent acceleration in death rate; (iii) lowering temperature from 25 degrees C to 22 degrees C retards the onset of age-related locomotor and learning deficits; and (iv) lowering temperature from 25 degrees C to 22 degrees C reduces the accumulation of the age-related marker lipofuscin. We conclude that lowering water temperature is a simple experimental manipulation which retards the rate of age-related damage accumulation in this short-lived species.
Full-textDOI: · Available from: Alessandro Cellerino, Sep 26, 2015
- SourceAvailable from: Margarita I Concha
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- "Another example is the phytoestrogen Quercetin, which is contained in green tea, onions, and apples (Ross and Kasum, 2002) and has also cardioprotective (Brookes et al., 2002), anticarcinogenic (Casagrande and Darbon, 2001), antioxidant (Heo and Lee, 2004), and antiapoptotic (Formica and Regelson, 1995) properties (Bureau et al., 2008). Rsv and Quercetin, which are known activators of Sirt1 (NAD + -dependent histone deacetylase) and AMPK (Cantó et al., 2010), have the ability to block agedependent decline in locomotor activity and memory (Valenzano et al., 2006). Rsv delays axonal degeneration after injury (Araki et al., 2004), blocks accumulation of A peptide in vitro (Han et al., 2004), and provides protection from brain ischemia in both adult and neonatal rodents (Wang et al., 2002). "
ABSTRACT: Herpes simplex virus type 1 (HSV-1) is ubiquitous and is able to establish a lifelong persistent latent infection in neurons of infected individuals. It has been estimated that in approximately 70% of the population over 50 years old, the virus enters the brain and infects neurons, and possibly undergoes recurrent reactivation episodes during lifetime, especially in immunodepressed individuals. We previously showed that the sensors AMP-dependent kinase (AMPK) and Sirtuin 1 (Sirt1), involved in survival pathways and neuroprotection, were affected during the course of HSV-1 infection. To evaluate if natural activators of the AMPK/Sirt1 axis, such as Resveratrol and Quercetin could reduce viral propagation and/or counteract the effects of neuronal infection we analyzed progeny virion production, neuronal viability and neurodegenerative events during HSV-1 infection. We found that the activators of AMPK/Sirt1 axis, increased the viability of infected neurons, significantly reduced the viral titer in the supernatant and the expression of viral genes. More importantly, pretreatment of neurons with Resveratrol or Quercetin significantly reduced the levels of caspase-3 cleaved- and hyperphosphorylated tau associated with HSV-1 infection. These results suggest that activators of the AMPK/Sirt1 axis could be potentially useful in reducing the risk of HSV-1 productive infection in neurons and the cellular damage associated with reactivation episodes. Copyright © 2015. Published by Elsevier B.V.Virus Research 05/2015; 205. DOI:10.1016/j.virusres.2015.05.015 · 2.32 Impact Factor
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- "Changes in temperature modulate ageing in C. elegans and D. melanogaster (Mair et al., 2003; Xiao et al., 2013), as well as in mice (Conti et al., 2006). Nothobranchius furzeri and N. rachovii ageing rate and overall survival in captivity can be modified by water temperature (Valenzano et al., 2006a; Hsu & Chiu, 2009), with lower temperature increasing lifespan, slowing down the appearance of ageing biomarkers, reducing adult body size and delaying the age-dependent decline in spontaneous activity. "
ABSTRACT: African annual fishes from the genus Nothobranchius are small teleosts that inhabit temporary water bodies subject to annual desiccation due to the alternation of the monsoon seasons. Given their unique biology, these fish have emerged as a model taxon in several biological disciplines. Their increasing popularity stems from the extremely short lifespan that is the result of their specific life-history adaptations and is retained under laboratory conditions. Nothobranchius furzeri, the most popular laboratory species, is the vertebrate species with the shortest lifespan recorded in captivity. In the laboratory, adults of different Nothobranchius species and populations live between 3 and 18 months and, notably, there is a negative correlation between the captive lifespan of a species and the aridity of their habitat. Their short lifespan is coupled to rapid age-dependent functional decline and expression of cellular and molecular changes comparable to those observed in other vertebrates, including humans. The recent development of transgenesis in this species makes it possible to insert specific constructs into their genome, and the establishment of transgenic lines is facilitated by their very rapid generation time, which can be as short as 1 month. This makes Nothobranchius species particularly suited for investigating biological and molecular aspects of ageing and ageing-associated dysfunctions. At the same time, they also represent a unique model taxon to investigate the evolution of life-history adaptations and their genetic architecture. We review their natural history, including phylogenetic relationships, distribution in relation to habitat conditions and natural selection for differential longevity, population structure and demography, and life cycle with emphasis on diapause that may occur at three stages during embryonic development. We further critically evaluate their use as a laboratory model for understanding the evolution of a rapid ageing rate and its consequences for other life-history traits, for cellular, molecular and integrative traits associated with the ageing process, high incidence of neoplasias, their utility for genome-wide gene-expression studies, and as a model for quantitative genetics. We summarize recent achievements in fostering Nothobranchius species as a widely applicable model system, including an annotated transcriptome, successful transgenesis, and existence of viable inbred lines. We compare the conditions they experience in the wild and in captivity and suggest that they are an ideal taxon to investigate natural genetic variation in a laboratory setting. We conclude that Nothobranchius species - and N. furzeri in particular - could become a unique model taxon that bridges interests in ecological and biomedical research. We hope that a conceptual and methodological integration of these two branches of biology will provide important new insights. © 2015 The Authors. Biological Reviews published by John Wiley & Sons Ltd on behalf of Cambridge Philosophical Society.Biological Reviews 04/2015; DOI:10.1111/brv.12183 · 9.67 Impact Factor
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- "Gotthard et al., 1999). Furthermore, higher latitudes are associated with cooler temperatures and, consequently, a slower rate of living, as shown for various animals such as fish and flies (Valenzano et al., 2006; Conti, 2008). In cold areas animals often hibernate. "
ABSTRACT: AimLongevity is an important life-history trait, directly linked to the core attributes of fitness (reproduction and survival), yet large-scale comparative studies quantifying its implications for the ecology and life history of ectotherms are scarce. We tested the allometry of longevity in squamates and the tuatara, and determined how longevity is related to key environmental characteristics and life-history traits. Predictions based on life-history theory are expected to hold true for ectotherms, similarly to mammals and birds.LocationWorld-wide.Methods We assembled from the literature a dataset of the maximum longevities of more than a thousand squamate species, representing c. 10% of their known species diversity, their phylogenetic relationships and multiple life-history and ecological variables. Correcting for phylogeny, we modelled the link between squamate longevity and both key life-history traits, such as body mass and age at first reproduction, and important environmental factors, such as latitude and primary productivity within species distributional ranges.ResultsLarge-bodied species live for longer than small ones, but body size explains far less of the variance in longevity than it does in mammals and birds. Accounting for body size, squamate brood frequency is negatively correlated with longevity, while age at first reproduction is positively correlated with longevity. This points to a continuum of slow-to-fast life-history strategies. Squamates in high latitudes and cold regions live for longer, probably because a shorter season of activity translates to slower development, older age at first reproduction and hence to increased longevity. Individuals live longer in captivity than in the wild. Herbivorous and omnivorous squamates live for longer than carnivorous ones. We postulate that low-quality nutrition reduces growth rates, promotes a relative decline in reproductive rates and thus prolongs life.Main conclusionsOur results support key predictions from life-history theory and suggest that reproducing more slowly and at older ages, being herbivorous and, plausibly, lowering metabolism, result in increased longevity.Global Ecology and Biogeography 04/2015; 24(4). DOI:10.1111/geb.12244 · 6.53 Impact Factor